Cited 3 times in

89 4

Buddlejasaponin IV induces cell cycle arrest at G2/M phase and apoptosis in immortalized human oral keratinocytes.

Authors
 Young Sun Hwang ; Won-Yoon Chung ; Kwang-Kyun Park ; Eun-Cheol Kim ; Hee-Juhn Park ; Jin Kim 
Citation
 Phytotherapy Research, Vol.25(10) : 1503~1510, 2011 
Journal Title
 Phytotherapy Research 
ISSN
 0951-418X 
Issue Date
2011
Abstract
Buddlejasaponin IV (BS-IV), a major component of Pleurospermum kamtschaticum, exerts antiinflammatory and cytotoxic effects against cancer cells. The study investigated whether BS-IV could prevent oral carcinogenesis by inhibiting the growth of immortalized human oral keratinocytes (IHOKs). BS-IV reduced cell viability and induced cell cycle arrest at G2/M phase and apoptotic morphological changes in IHOKs. BS-IV inhibited the levels of cyclin B1, Cdc2 and Cdc25C, but enhanced Chk2 phosphorylation. The increased levels of pRb and p21 protein and the activation of p53 were also noted in BS-IV-treated IHOKs. In addition, BS-IV induced cytochrome c release from mitochondria by reducing antiapoptotic Bcl-2 levels and increasing pro-apoptotic Bax levels. BS-IV treatment resulted in the activation of caspase-9 and caspase-3. PARP cleavage was also clearly observed in the BS-IV-treated IHOKs. Furthermore, the expression of the Fas death receptor and Fas ligand was induced and procaspase-8 level was suppressed by BS-IV treatment. Taken together, BS-IV treatment inhibited the growth of IHOK cells via the induction of p53-dependent cell cycle arrest at the G2/M phase and apoptosis via both mitochondrial-dependent and death receptor-mediated pathways. Thus, BS-IV can be considered an excellent candidate for a chemopreventive agent to block the progression of HPV-induced oral carcinogenesis
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94325
DOI
10.1002/ptr.3406
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Pathology
1. 연구논문 > 5. Research Institutes > Oral Cancer Research Institute
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://onlinelibrary.wiley.com/doi/10.1002/ptr.3406/abstract
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse