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Hypoxia-induced expression of VEGF in the organotypic spinal cord slice culture

Authors
 Sung Su An  ;  William A. Pennant  ;  Yoon Ha  ;  Jin Soo Oh  ;  Hyo Jin Kim  ;  So-Jung Gwak  ;  Do Heum Yoon  ;  Keung Nyun Kim 
Citation
 NEUROREPORT, Vol.22(2) : 55-60, 2011 
Journal Title
 NEUROREPORT 
ISSN
 0959-4965 
Issue Date
2011
MeSH
Animals ; Astrocytes/metabolism ; Cell Differentiation ; Cell Proliferation ; Gene Expression ; Hypoxia/genetics ; Hypoxia/metabolism* ; Neurons ; Rats ; Rats, Sprague-Dawley ; Spinal Cord/metabolism* ; Transfection ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism*
Keywords
gene therapy ; hypoxia-inducible gene expression system ; organotypic spinal cord slice culture
Abstract
We used the erythropoietin enhancer and Simian virus-40 promoter to create a hypoxia-inducible gene expression system to investigate the effect of vascular endothelial growth factor (VEGF) gene therapy on neuroprotection and neurogenesis in organotypic spinal cord slice culture. The organotypic spinal cord slice culture transfected with pEpo-SV-VEGF expressed the highest amount of VEGF under hypoxic conditions and showed decreased apoptosis and increased proliferation, and evidence of neurogenesis. Our results show that the hypoxia-induced VEGF expression in an organotypic spinal cord slice culture may lead to an optimal treatment for spinal cord injury.
Full Text
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00001756-201101260-00002&LSLINK=80&D=ovft
DOI
10.1097/WNR.0b013e3283418b00
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Gwak, So Jung(곽소정)
Kim, Keung Nyun(김긍년)
Kim, Hyo Jin(김효진)
An, Sung Su(안성수)
Oh, Jin Soo(오진수)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Ha, Yoon(하윤)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94171
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