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Effects of HB-EGF and epiregulin on wound healing of gingival cells in vitro.

 JM Kim  ;  EJ Bak  ;  JY Chang  ;  S-T Kim  ;  W-S Park  ;  Y-J Yoo  ;  J-H Cha 
 ORAL DISEASES, Vol.17(8) : 785-793, 2011 
Journal Title
Issue Date
Cell Culture Techniques ; Cell Movement/drug effects ; Cell Movement/physiology ; Cell Proliferation/drug effects ; Epidermal Growth Factor/analysis* ; Epidermal Growth Factor/pharmacology ; Epiregulin ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Gingiva/cytology ; Gingiva/drug effects ; Gingiva/metabolism* ; Heparin/analysis* ; Heparin/pharmacology ; Heparin-binding EGF-like Growth Factor ; Humans ; Intercellular Signaling Peptides and Proteins/analysis* ; Intercellular Signaling Peptides and Proteins/pharmacology ; RNA, Messenger/analysis ; Real-Time Polymerase Chain Reaction ; Receptor, Epidermal Growth Factor/analysis ; Receptor, ErbB-2/analysis ; Receptor, ErbB-3/analysis ; Receptors, Cell Surface/analysis* ; Receptors, Cell Surface/physiology ; Wound Healing/physiology
heparin-binding epidermal growth factor-like growth factor ; epiregulin ; gingival tissue ; wound healing
OBJECTIVE: Gingival wound healing is important to periodontal disease and surgery. This in vitro study was conducted to assess the manner in which heparin-binding epidermal growth factor-like growth factor (HB-EGF) and epiregulin cooperatively participate in the wound-healing process in the gingival epithelial and fibroblast cells of the oral mucosa.

MATERIAL AND METHODS: Gingival epithelium and fibroblast were separated from gingival tissue biopsies and prepared to primary cultures. The changes in the mRNA expression were evaluated via real-time PCR. The effects on cell proliferation, migration, and repopulation were evaluated in vitro.

RESULTS: The different regulation of expressions of HB-EGF, epiregulin, and epidermal growth factor receptors was observed over time and with different gingival cell types. HB-EGF exerted a cell migration-inducing effect on both epithelial and fibroblast cells, whereas epiregulin did not. Both growth factors functioned as mitogens for epithelial cell proliferation, but not for fibroblast proliferation. HB-EGF strongly promoted epithelial cell repopulation and mildly promoted fibroblast repopulation, whereas epiregulin promoted only fibroblast repopulation.

CONCLUSION: These results indicated that both growth factors might function importantly in the wound-healing process of human gingival tissue via the different regulation of the expression, cell migration, proliferation, and repopulation.
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Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Prosthodontics (보철과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Tae(김성태)
Kim, Jin Moon(김진문)
Park, Wonse(박원서) ORCID logo https://orcid.org/0000-0002-2081-1156
Bak, Eun-Jung(박은정) ORCID logo https://orcid.org/0000-0002-7976-8594
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Cha, Jeong Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
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