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Albumin-like protein is the major protein constituent of luminal fluid in the human endolymphatic sac.

 Sung Huhn Kim  ;  Un-Kyoung Kim  ;  Won-Sang Lee  ;  Jinwoong Bok  ;  Jung-Whan Song  ;  Je Kyung Seong  ;  Jae Young Choi 
 PLOS ONE, Vol.6(6) : e21656, 2011 
Journal Title
Issue Date
Adolescent ; Adult ; Albumins/metabolism* ; Child ; Child, Preschool ; Electrophoresis, Gel, Two-Dimensional ; Endolymphatic Duct/metabolism ; Endolymphatic Sac/metabolism* ; Female ; Humans ; Male ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
The endolymphatic sac (ES) is an inner ear organ that is connected to the cochleo-vestibular system through the endolymphatic duct. The luminal fluid of the ES contains a much higher concentration of proteins than any other compartment of the inner ear. This high protein concentration likely contributes to inner ear fluid volume regulation by creating an osmotic gradient between the ES lumen and the interstitial fluid. We characterized the protein profile of the ES luminal fluid of patients (n = 11) with enlarged vestibular aqueducts (EVA) by proteomics. In addition, we investigated differences in the protein profiles between patients with recent hearing deterioration and patients without hearing deterioration. The mean total protein concentration of the luminal fluid was 554.7±94.6 mg/dl. A total of 58 out of 517 spots detected by 2-DE were analyzed by MALDI-TOF MS. The protein profile of the luminal fluid was different from the profile of plasma. Proteins identified from 29 of the spots were also present in the MARC-filtered human plasma; however, the proteins identified from the other 25 spots were not detected in the MARC-filtered human plasma. The most abundant protein in the luminal fluid was albumin-like proteins, but most of them were not detected in MARC-filtered human plasma. The concentration of albumin-like proteins was higher in samples from patients without recent hearing deterioration than in patients with recent hearing deterioration. Consequently, the protein of ES luminal fluid is likely to be originated from both the plasma and the inner ear and considering that inner ear fluid volumes increase abnormally in patients with EVA following recent hearing deterioration, it is tempting to speculate that albumin-like proteins may be involved in the regulation of inner ear fluid volume through creation of an osmotic gradient during pathological conditions such as endolymphatic hydrops.
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1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Huhn(김성헌)
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
Lee, Won Sang(이원상)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
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