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Potential of nucleofected human MSCs for insulin secretion.

Authors
 Jae Hyung Kim ; Kyoo-Ho Shin ; Hwal Suh ; Tian Zhu Li 
Citation
 Journal of Tissue Enginerring and Regenerative Medicine, Vol.5(10) : 761~769, 2011 
Journal Title
 Journal of Tissue Enginerring and Regenerative Medicine 
ISSN
 1932-6254 
Issue Date
2011
Abstract
The goal of this experiment was to generate insulin-producing human mesenchymal stem cells (hMSCs) as a therapeutic source for type I diabetes mellitus, which is caused by insulin deficiency due to the destruction of islet β cells. In various trials for the treatment of type I diabetes, cell-based therapy using adult stem cells is considered to be one of the most useful candidates for the treatment. In this experiment, a non-viral method called nucleofection was used to transfect hMSCs with pEGFP-C2 and furin-cleavable human preproinsulin gene (hPPI) to produce insulin-secreting cells as surrogate β cells. Transfection efficiency was determined using flow cytometry analysis. Expression and production of insulin were tested using RT-PCR and ELISA. The expression, production and maturation of insulin from the genetically engineered hMSCs showed an increase when compared with a non-transfected control group. Insulin expression from hMSCs using nucleofection in this study has shown the potential for type I diabetes therapy. For further study, an evaluation for in vivo experiments and clinical applications must be supplemented.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93773
DOI
10.1002/term.371
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Medical Engineering
1. 연구논문 > 1. College of Medicine > Dept. of Orthopedic Surgery
Yonsei Authors
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Link
 http://onlinelibrary.wiley.com/doi/10.1002/term.371/abstract
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