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Potential of nucleofected human MSCs for insulin secretion.

Authors
 Jae Hyung Kim  ;  Kyoo-Ho Shin  ;  Tian Zhu Li  ;  Hwal Suh 
Citation
 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.5(10) : 761-769, 2011 
Journal Title
 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 
ISSN
 1932-6254 
Issue Date
2011
MeSH
Adult ; Cells, Cultured ; Diabetes Mellitus, Type 1/genetics ; Diabetes Mellitus, Type 1/metabolism ; Diabetes Mellitus, Type 1/therapy ; Gene Expression ; Humans ; Insulin/genetics ; Insulin/secretion* ; Insulin-Secreting Cells/cytology ; Insulin-Secreting Cells/metabolism ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/secretion* ; Protein Precursors/genetics ; Protein Precursors/secretion* ; Recombinant Proteins/genetics ; Recombinant Proteins/secretion ; Transfection
Keywords
type I diabetes ; cell-based gene therapy ; mesenchymal stem cells ; nucleofection ; surrogate β cells ; insulin secretion
Abstract
The goal of this experiment was to generate insulin-producing human mesenchymal stem cells (hMSCs) as a therapeutic source for type I diabetes mellitus, which is caused by insulin deficiency due to the destruction of islet β cells. In various trials for the treatment of type I diabetes, cell-based therapy using adult stem cells is considered to be one of the most useful candidates for the treatment. In this experiment, a non-viral method called nucleofection was used to transfect hMSCs with pEGFP-C2 and furin-cleavable human preproinsulin gene (hPPI) to produce insulin-secreting cells as surrogate β cells. Transfection efficiency was determined using flow cytometry analysis. Expression and production of insulin were tested using RT-PCR and ELISA. The expression, production and maturation of insulin from the genetically engineered hMSCs showed an increase when compared with a non-transfected control group. Insulin expression from hMSCs using nucleofection in this study has shown the potential for type I diabetes therapy. For further study, an evaluation for in vivo experiments and clinical applications must be supplemented.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/term.371/abstract
DOI
10.1002/term.371
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Hyung(김재형)
Li, Tian Zhu(리천주)
Suh, Hwal(서활)
Shin, Kyoo Ho(신규호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93773
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