Cited 11 times in
Potential of nucleofected human MSCs for insulin secretion.
DC Field | Value | Language |
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dc.contributor.author | 김재형 | - |
dc.contributor.author | 리천주 | - |
dc.contributor.author | 서활 | - |
dc.contributor.author | 신규호 | - |
dc.date.accessioned | 2014-12-20T16:59:48Z | - |
dc.date.available | 2014-12-20T16:59:48Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1932-6254 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93773 | - |
dc.description.abstract | The goal of this experiment was to generate insulin-producing human mesenchymal stem cells (hMSCs) as a therapeutic source for type I diabetes mellitus, which is caused by insulin deficiency due to the destruction of islet β cells. In various trials for the treatment of type I diabetes, cell-based therapy using adult stem cells is considered to be one of the most useful candidates for the treatment. In this experiment, a non-viral method called nucleofection was used to transfect hMSCs with pEGFP-C2 and furin-cleavable human preproinsulin gene (hPPI) to produce insulin-secreting cells as surrogate β cells. Transfection efficiency was determined using flow cytometry analysis. Expression and production of insulin were tested using RT-PCR and ELISA. The expression, production and maturation of insulin from the genetically engineered hMSCs showed an increase when compared with a non-transfected control group. Insulin expression from hMSCs using nucleofection in this study has shown the potential for type I diabetes therapy. For further study, an evaluation for in vivo experiments and clinical applications must be supplemented. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 761~769 | - |
dc.relation.isPartOf | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Diabetes Mellitus, Type 1/genetics | - |
dc.subject.MESH | Diabetes Mellitus, Type 1/metabolism | - |
dc.subject.MESH | Diabetes Mellitus, Type 1/therapy | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Insulin/genetics | - |
dc.subject.MESH | Insulin/secretion* | - |
dc.subject.MESH | Insulin-Secreting Cells/cytology | - |
dc.subject.MESH | Insulin-Secreting Cells/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mesenchymal Stem Cell Transplantation | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology | - |
dc.subject.MESH | Mesenchymal Stromal Cells/secretion* | - |
dc.subject.MESH | Protein Precursors/genetics | - |
dc.subject.MESH | Protein Precursors/secretion* | - |
dc.subject.MESH | Recombinant Proteins/genetics | - |
dc.subject.MESH | Recombinant Proteins/secretion | - |
dc.subject.MESH | Transfection | - |
dc.title | Potential of nucleofected human MSCs for insulin secretion. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학) | - |
dc.contributor.googleauthor | Jae Hyung Kim | - |
dc.contributor.googleauthor | Kyoo-Ho Shin | - |
dc.contributor.googleauthor | Tian Zhu Li | - |
dc.contributor.googleauthor | Hwal Suh | - |
dc.identifier.doi | 10.1002/term.371 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01338 | - |
dc.contributor.localId | A01924 | - |
dc.contributor.localId | A02086 | - |
dc.contributor.localId | A00873 | - |
dc.relation.journalcode | J01911 | - |
dc.identifier.eissn | 1932-7005 | - |
dc.identifier.pmid | 22002919 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/term.371/abstract | - |
dc.subject.keyword | type I diabetes | - |
dc.subject.keyword | cell-based gene therapy | - |
dc.subject.keyword | mesenchymal stem cells | - |
dc.subject.keyword | nucleofection | - |
dc.subject.keyword | surrogate β cells | - |
dc.subject.keyword | insulin secretion | - |
dc.contributor.alternativeName | Kim, Jae Hyung | - |
dc.contributor.alternativeName | Li, Tian Zhu | - |
dc.contributor.alternativeName | Suh, Hwal | - |
dc.contributor.alternativeName | Shin, Kyoo Ho | - |
dc.contributor.affiliatedAuthor | Li, Tian Zhu | - |
dc.contributor.affiliatedAuthor | Suh, Hwal | - |
dc.contributor.affiliatedAuthor | Shin, Kyoo Ho | - |
dc.contributor.affiliatedAuthor | Kim, Jae Hyung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 5 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 761 | - |
dc.citation.endPage | 769 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.5(10) : 761-769, 2011 | - |
dc.identifier.rimsid | 28450 | - |
dc.type.rims | ART | - |
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