Cited 26 times in
The extracellular signal-regulated kinase mitogen-activated protein kinase/ribosomal S6 protein kinase 1 cascade phosphorylates cAMP response element-binding protein to induce MUC5B gene expression via D-prostanoid receptor signaling.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박보령 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 유정윤 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 윤호근 | - |
dc.contributor.author | 이상남 | - |
dc.contributor.author | 최연호 | - |
dc.date.accessioned | 2014-12-20T16:59:30Z | - |
dc.date.available | 2014-12-20T16:59:30Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93763 | - |
dc.description.abstract | Mucus hypersecretion is a prominent feature of respiratory diseases, and MUC5B is a major airway mucin. Mucin gene expression can be affected by inflammatory mediators, including prostaglandin (PG) D(2,) an inflammatory mediator synthesized by hematopoietic PGD synthase (H-PGDS). PGD(2) binds to either D-prostanoid receptor (DP1) or chemoattractant receptor homologous molecule expressed on T-helper type 2 cells (CRTH2). We investigated the mechanisms by which PGD(2) induces MUC5B gene expression in airway epithelial cells. Western blot analysis showed that H-PGDS was highly expressed in nasal polyps. Similar results were obtained for PGD(2) expression. In addition, we could clearly detect the expressions of both H-PGDS and DP1 in nasal epithelial cells but not CRTH2. We demonstrated that PGD(2) increased MUC5B gene expression in normal human nasal epithelial cells as well as in NCI-H292 cells in vitro. S5751, a DP1 antagonist, inhibited PGD(2)-induced MUC5B expression, whereas a CRTH2 antagonist (OC0459) did not. These data suggest that PGD(2) induced MUC5B expression via DP1. Pretreatment with extracellular signal-regulated kinase (ERK) inhibitor (PD98059) blocked both PGD(2)-induced ERK mitogen-activated protein kinase (MAPK) activation and MUC5B expression. Proximity ligation assays showed direct interaction between RSK1 and cAMP response element-binding protein (CREB). Stimulation with PGD(2) caused an increase in intracellular cAMP levels, whereas intracellular Ca(2+) did not have such an effect. PGD(2)-induced MUC5B mRNA levels were regulated by CREB via direct interaction with two cAMP-response element sites (-921/-914 and -900/-893). Finally, we demonstrated that PGD(2) can induce MUC5B overproduction via ERK MAPK/RSK1/CREB signaling and that DP1 receptor may have suppressive effects in controlling MUC5B overproduction in the airway. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 34199~34214 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cyclic AMP/metabolism | - |
dc.subject.MESH | Cyclic AMP Response Element-Binding Protein/metabolism* | - |
dc.subject.MESH | Enzyme Activation/drug effects | - |
dc.subject.MESH | Enzyme Activation/physiology | - |
dc.subject.MESH | Epithelial Cells/cytology | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism* | - |
dc.subject.MESH | Flavonoids/pharmacology | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Gene Expression Regulation/physiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intramolecular Oxidoreductases/metabolism | - |
dc.subject.MESH | Lipocalins/metabolism | - |
dc.subject.MESH | MAP Kinase Signaling System/drug effects | - |
dc.subject.MESH | MAP Kinase Signaling System/physiology | - |
dc.subject.MESH | Mucin-5B/biosynthesis* | - |
dc.subject.MESH | Phosphorylation/drug effects | - |
dc.subject.MESH | Phosphorylation/physiology | - |
dc.subject.MESH | Prostaglandin D2/metabolism | - |
dc.subject.MESH | Receptors, Immunologic/antagonists & inhibitors | - |
dc.subject.MESH | Receptors, Immunologic/metabolism | - |
dc.subject.MESH | Receptors, Prostaglandin/antagonists & inhibitors | - |
dc.subject.MESH | Receptors, Prostaglandin/metabolism* | - |
dc.subject.MESH | Respiratory Mucosa/cytology | - |
dc.subject.MESH | Respiratory Mucosa/metabolism* | - |
dc.subject.MESH | Response Elements/physiology | - |
dc.subject.MESH | Ribosomal Protein S6 Kinases, 90-kDa/metabolism* | - |
dc.subject.MESH | Thiophenes/pharmacology | - |
dc.title | The extracellular signal-regulated kinase mitogen-activated protein kinase/ribosomal S6 protein kinase 1 cascade phosphorylates cAMP response element-binding protein to induce MUC5B gene expression via D-prostanoid receptor signaling. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Yeon Ho Choi | - |
dc.contributor.googleauthor | Sang-Nam Lee | - |
dc.contributor.googleauthor | Hiroki Aoyagi | - |
dc.contributor.googleauthor | Yasundo Yamasaki | - |
dc.contributor.googleauthor | Jung-Yoon Yoo | - |
dc.contributor.googleauthor | Boryung Park | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Ho-Geun Yoon | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1074/jbc.M111.247684 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01481 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A02625 | - |
dc.contributor.localId | A02813 | - |
dc.contributor.localId | A04110 | - |
dc.contributor.localId | A02502 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 21832046 | - |
dc.subject.keyword | DP1 | - |
dc.subject.keyword | MUC5B | - |
dc.subject.keyword | Prostaglandin D2 | - |
dc.contributor.alternativeName | Park, Bo Ryung | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Yoo, Jung Yoon | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Yoon, Ho Geun | - |
dc.contributor.alternativeName | Lee, Sang Nam | - |
dc.contributor.alternativeName | Choi, Yeon Ho | - |
dc.contributor.affiliatedAuthor | Park, Bo Ryung | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Lee, Sang Nam | - |
dc.contributor.affiliatedAuthor | Choi, Yeon Ho | - |
dc.contributor.affiliatedAuthor | Yoo, Jung Yoon | - |
dc.contributor.affiliatedAuthor | 유정윤 | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 286 | - |
dc.citation.number | 39 | - |
dc.citation.startPage | 34199 | - |
dc.citation.endPage | 34214 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.286(39) : 34199-34214, 2011 | - |
dc.identifier.rimsid | 28447 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.