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Effect of diode laser on proliferation and differentiation of PC12 cells

Authors
 Kensuke Saito ; Sadamitsu Hashimoto ; Han-Sung Jung ; Masaki Shimono ; Kan-Ichi Nakagawa 
Citation
 Bulletin of Tokyo Dental College, Vol.52(2) : 95~102, 2011 
Journal Title
 Bulletin of Tokyo Dental College 
ISSN
 0040-8891 
Issue Date
2011
Abstract
This study investigated the effects of diode (GaAlAs) laser irradiation at an effective energy density of 5 or 20 J/cm(2) on cell growth factor-induced differentiation and proliferation in pheochromocytoma cells (PC12 cells), and whether those effects were related to activation of the p38 pathway. Laser irradiation at 20 J/cm(2) significantly decreased the number of PC12 cells, while no difference was seen between the 5 J/cm(2) group and the control group (p<0.05). Western blotting revealed marked expression of neurofilament and β-tubulin, indicating greater neurite differentiation in the irradiation groups than in the control group at 48 hr. Irradiation also enhanced expression of phospho-p38. The decrease in number of cells after laser irradiation was accelerated by p38 inhibitor, while neurite differentiation was up-regulated by laser irradiation, even when the p38 pathway was blocked. This suggests that laser irradiation up-regulated neurite differentiation in PC12 cells involving p38 and another pathway.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93739
DOI
10.2209/tdcpublication.52.95
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
Yonsei Authors
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