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Cornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor γ and insulin sensitization

 Daeyoung Kim  ;  Kwang-Kyun Park  ;  Sang Kook Lee  ;  Seung-Eun Lee  ;  Jae-Kwan Hwang 
 JOURNAL OF ETHNOPHARMACOLOGY, Vol.133(2) : 803-809, 2011 
Journal Title
Issue Date
3T3-L1 Cells ; Adipogenesis/drug effects ; Animals ; COS Cells ; Cercopithecus aethiops ; Cornus* ; Diabetes Mellitus ; Type 2/drug therapy ; Diabetes Mellitus ; Type 2/metabolism ; Ethnopharmacology ; Glucose/metabolism ; Humans ; Hyperglycemia/drug therapy ; Hyperglycemia/metabolism ; Insulin/metabolism* ; Ligands ; Medicine ; Korean Traditional ; Mice ; PPAR gamma/metabolism* ; Phytotherapy ; Plant Preparations/administration & dosage ; Plant Preparations/pharmacology* ; Plants ; Medicinal ; Republic of Korea ; Signal Transduction/drug effects
Peroxisome proliferator-activated receptor γ (PPARγ) ; Cornus kousa F.Buerger ex Miquel ; Oriental wild plants ; Type 2 diabetes ; Insulin resistance
AIM OF THE STUDY: Cornus kousa F.Buerger ex Miquel, an oriental medicinal plant, has been traditionally used for the treatment of hyperglycemia, but its molecular mechanism remains unknown. The goal of this study was to investigate the peroxisome proliferator-activated receptor γ (PPARγ) ligand-binding activity of Cornus kousa and to determine the effects of Cornus kousa on insulin sensitization in 3T3-L1 cells for the treatment of type 2 diabetes. MATERIALS AND METHODS: PPARγ luciferase transactivation assay was used to evaluate the PPARγ ligand-binding activity of Cornus kousa leaf extract. Western blot analysis, oil Red O staining, and glucose uptake assay were performed to evaluate PPARγ agonistic activity and insulin sensitizing effects of Cornus kousa leaf extract (CKE) in 3T3-L1 cells. RESULTS: CKE increased PPARγ ligand-binding activity in a dose-dependent manner. In addition, CKE enhanced adipogenesis and the expression of PPARγ target proteins, including glucose transporter 4 (GLUT4) and adiponectin, as well as proteins involved in adipogenesis, including PPARγ and CCAAT/enhancer binding protein α (C/EBPα) in 3T3-L1 adipocytes. Furthermore, CKE led to significant induction of glucose uptake and stimulated insulin signaling, but not to activation of AMP-activated protein kinase (AMPK) signaling. The enhanced glucose uptake by CKE were abolished by treatment with bisphenol a diglycidyl ether (BADGE), a PPARγ antagonist, or LY294002, an inhibitor of phosphoinositide 3-kinase (PI3K), but not by compound C, an AMPK inhibitor. CONCLUSION: Consistent with the high PPARγ ligand-binding activity, CKE increased glucose uptake through PPARγ activation and insulin signaling. These results suggest that CKE could have pharmacological effects for the treatment of hyperglycemia and type 2 diabetes
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2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Kwang Kyun(박광균)
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