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Cornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor γ and insulin sensitization

DC FieldValueLanguage
dc.contributor.author박광균-
dc.date.accessioned2014-12-20T16:47:08Z-
dc.date.available2014-12-20T16:47:08Z-
dc.date.issued2011-
dc.identifier.issn0378-8741-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/93373-
dc.description.abstractAIM OF THE STUDY: Cornus kousa F.Buerger ex Miquel, an oriental medicinal plant, has been traditionally used for the treatment of hyperglycemia, but its molecular mechanism remains unknown. The goal of this study was to investigate the peroxisome proliferator-activated receptor γ (PPARγ) ligand-binding activity of Cornus kousa and to determine the effects of Cornus kousa on insulin sensitization in 3T3-L1 cells for the treatment of type 2 diabetes. MATERIALS AND METHODS: PPARγ luciferase transactivation assay was used to evaluate the PPARγ ligand-binding activity of Cornus kousa leaf extract. Western blot analysis, oil Red O staining, and glucose uptake assay were performed to evaluate PPARγ agonistic activity and insulin sensitizing effects of Cornus kousa leaf extract (CKE) in 3T3-L1 cells. RESULTS: CKE increased PPARγ ligand-binding activity in a dose-dependent manner. In addition, CKE enhanced adipogenesis and the expression of PPARγ target proteins, including glucose transporter 4 (GLUT4) and adiponectin, as well as proteins involved in adipogenesis, including PPARγ and CCAAT/enhancer binding protein α (C/EBPα) in 3T3-L1 adipocytes. Furthermore, CKE led to significant induction of glucose uptake and stimulated insulin signaling, but not to activation of AMP-activated protein kinase (AMPK) signaling. The enhanced glucose uptake by CKE were abolished by treatment with bisphenol a diglycidyl ether (BADGE), a PPARγ antagonist, or LY294002, an inhibitor of phosphoinositide 3-kinase (PI3K), but not by compound C, an AMPK inhibitor. CONCLUSION: Consistent with the high PPARγ ligand-binding activity, CKE increased glucose uptake through PPARγ activation and insulin signaling. These results suggest that CKE could have pharmacological effects for the treatment of hyperglycemia and type 2 diabetes-
dc.description.statementOfResponsibilityopen-
dc.format.extent803~809-
dc.relation.isPartOfJOURNAL OF ETHNOPHARMACOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3T3-L1 Cells-
dc.subject.MESHAdipogenesis/drug effects-
dc.subject.MESHAnimals-
dc.subject.MESHCOS Cells-
dc.subject.MESHCercopithecus aethiops-
dc.subject.MESHCornus*-
dc.subject.MESHDiabetes Mellitus-
dc.subject.MESHType 2/drug therapy-
dc.subject.MESHDiabetes Mellitus-
dc.subject.MESHType 2/metabolism-
dc.subject.MESHEthnopharmacology-
dc.subject.MESHGlucose/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHHyperglycemia/drug therapy-
dc.subject.MESHHyperglycemia/metabolism-
dc.subject.MESHInsulin/metabolism*-
dc.subject.MESHLigands-
dc.subject.MESHMedicine-
dc.subject.MESHKorean Traditional-
dc.subject.MESHMice-
dc.subject.MESHPPAR gamma/metabolism*-
dc.subject.MESHPhytotherapy-
dc.subject.MESHPlant Preparations/administration & dosage-
dc.subject.MESHPlant Preparations/pharmacology*-
dc.subject.MESHPlants-
dc.subject.MESHMedicinal-
dc.subject.MESHRepublic of Korea-
dc.subject.MESHSignal Transduction/drug effects-
dc.titleCornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor γ and insulin sensitization-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorDaeyoung Kim-
dc.contributor.googleauthorKwang-Kyun Park-
dc.contributor.googleauthorSang Kook Lee-
dc.contributor.googleauthorSeung-Eun Lee-
dc.contributor.googleauthorJae-Kwan Hwang-
dc.identifier.doi10.1016/j.jep.2010.11.007-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01429-
dc.relation.journalcodeJ01406-
dc.identifier.eissn1872-7573-
dc.identifier.pmid21070843-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0378874110007798-
dc.subject.keywordPeroxisome proliferator-activated receptor γ (PPARγ)-
dc.subject.keywordCornus kousa F.Buerger ex Miquel-
dc.subject.keywordOriental wild plants-
dc.subject.keywordType 2 diabetes-
dc.subject.keywordInsulin resistance-
dc.contributor.alternativeNamePark, Kwang Kyun-
dc.contributor.affiliatedAuthorPark, Kwang Kyun-
dc.rights.accessRightsnot free-
dc.citation.volume133-
dc.citation.number2-
dc.citation.startPage803-
dc.citation.endPage809-
dc.identifier.bibliographicCitationJOURNAL OF ETHNOPHARMACOLOGY, Vol.133(2) : 803-809, 2011-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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