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Genome-wide molecular characterization of mucinous colorectal adenocarcinoma using cDNA microarray analysis.

Authors
 Han Sang Kim  ;  Seung Hui Kang  ;  Chan Hee Park  ;  Woo Ick Yang  ;  Hei Cheul Jeung  ;  Hyun Cheol Chung  ;  Jae Kyung Roh  ;  Joong Bae Ahn  ;  Nam Kyu Kim  ;  Byung Soh Min  ;  Sun Young Rha 
Citation
 ONCOLOGY REPORTS, Vol.25(3) : 717-727, 2011 
Journal Title
ONCOLOGY REPORTS
ISSN
 1021-335X 
Issue Date
2011
MeSH
Adenocarcinoma, Mucinous/genetics* ; Adult ; Aged ; Aged, 80 and over ; Algorithms ; Cell Line, Tumor ; Colorectal Neoplasms/genetics* ; Female ; Gene Expression Profiling* ; Gene Expression Regulation, Neoplastic ; Genome, Human/genetics ; Humans ; Male ; Matched-Pair Analysis ; Middle Aged ; Oligonucleotide Array Sequence Analysis* ; Validation Studies as Topic
Abstract
Mucinous colorectal carcinoma exhibits distinct clinicopathological features compared to non-mucinous colorectal carcinoma. Previous studies have discovered several molecular genetic features in mucinous colorectal carcinomas, but have limitations as they are confined to a small number of molecules. To understand the mucinous colorectal carcinoma system, this study was designed to identify genes that are differentially expressed in mucinous colorectal carcinoma compared to non-mucinous colorectal carcinoma using cDNA microarrays. cDNA microarray experiments were performed using human cDNA 17k chips with 25 mucinous and 27 non-mucinous cancer tissues. Differentially expressed genes (DEGs) were determined by Welch's t-test and more accurate classifiers were selected from the DEGs using the prediction analysis for microarrays (PAM) software package. Array results were validated using quantitative real-time RT-PCR. The identified gene set was functionally investigated through in silico analysis. Sixty-two DEGs were identified and the 50 highest ranking genes could be used to accurately classify mucinous and non-mucinous colorectal carcinomas. The identified gene set included up-regulated TFF1 (4-fold), AGR2 (3.3-fold), FSCN1 (2.2-fold), CD44 (1.5-fold) and down-regulated SLC26A3 (0.2-fold) in MC. TFF1, AGR2 and SLC26A3 were validated by quantitative real-time RT-PCR. The functions of these DEGs were related to tumorigenesis (14 genes), cell cycle progression (6 genes), invasion (2 genes), anti-apoptosis (7 genes), cell adhesion and proliferation (5 genes) and carbohydrate metabolism (3 genes). We suggest that MC has distinct molecular characteristics from NMC and therefore, that the expression signatures of DEGs may improve the understanding of molecular pathogenesis and clinical behaviors in MC.
Full Text
http://www.spandidos-publications.com/or/25/3/717
DOI
10.3892/or.2010.1126
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Seung Hui(강승희)
Kim, Nam Kyu(김남규) ORCID logo https://orcid.org/0000-0003-0639-5632
Kim, Han Sang(김한상) ORCID logo https://orcid.org/0000-0002-6504-9927
Roh, Jae Kyung(노재경)
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Min, Byung Soh(민병소) ORCID logo https://orcid.org/0000-0003-0180-8565
Park, Chan Hee(박찬희)
Ahn, Joong Bae(안중배) ORCID logo https://orcid.org/0000-0001-6787-1503
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93058
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