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Effect of (-)-epigallocatechin-3-gallate on maintaining the periodontal ligament cell viability of avulsed teeth: a preliminary study

Authors
 Im-Hee Jung  ;  Jeong-Ho Yun  ;  Ah-Ran Cho  ;  Chang-Sung Kim  ;  Won-Gyun Chung  ;  Seong-Ho Choi 
Citation
 Journal of Periodontal & Implant Science, Vol.41(1) : 10-16, 2011 
Journal Title
 Journal of Periodontal & Implant Science 
ISSN
 2503-3352 
Issue Date
2011
Abstract
PURPOSE: Avulsed tooth can be completely recovered, if sound periodontal ligament (PDL) of tooth is maintained. Although a lot of storage solutions have been explored for the better storage of avulsed tooth, there is a shortcoming that the preservation time is much short. On the other hand, there has been studies that (-)-epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in green tea, which is related to the anti inflammatory, antioxygenic, and antibacterial effects, allows the successful preservations of tissues and cells. This study evaluated the effect of EGCG on avulsed-teeth preservation of Beagle dogs for a period of time. METHODS: The atraumatically extracted teeth of Beagle dogs were washed and preserved with 0/10/100 µM of EGCG at the time of immediate, period 1 (4 days in EGCG-contained media and additional 1 day in EGCG-free media), period 2 (8 days in EGCG-contained media and additional 2 days in EGCG-free media) and period 3 (12 days in EGCG-contained media and additional 2 days in EGCG-free media). Then, the cell viabilities of preserved teeth was calculated by dividing optical density (OD) of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with OD of eosin assay to eliminate the measurement errors caused by the different tissue volumes. RESULTS: From the results, the immediately analyzed group presented the highest cell viability, and the rate of living cells on teeth surface decreased dependent on the preservation period. However, the 100 µM of EGCG-treated group showed statistically significant positive cell activity than EGCG-free groups throughout preservation periods. CONCLUSIONS: Our findings showed that 100 µM EGCG could maintain PDL cell viability of extracted tooth. These results suggest that although EGCG could not be a perfect additive for tooth preservation, it is able to postpone the period of tooth storage. However, further in-depth studies are required for more plausible use of EGCG.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/92711
DOI
10.5051/jpis.2011.41.1.10
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Institute for Periodontal Tissue Regeneration (치주조직재생연구소)
Yonsei Authors
김창성(Kim, Chang Sung) ; 윤정호(Yun, Jeong Ho) ; 정임희(Jung, Im Hee) ; 조아란(Cho, Ah Ran) ; 최성호(Choi, Seong Ho)
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