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Carbapenem-non-susceptible Acinetobacter baumannii of sequence type 92 or its single-locus variants with a G428T substitution in zone 2 of the rpoB gene

Authors
 Yangsoon Lee  ;  Jinhwa Lee  ;  Seok Hoon Jeong  ;  Jonghan Lee  ;  Il Kwon Bae  ;  Kyungwon Lee 
Citation
 Journal of Antimicrobial Chemotherapy, Vol.66(1) : 66-72, 2011 
Journal Title
 Journal of Antimicrobial Chemotherapy 
ISSN
 0305-7453 
Issue Date
2011
Abstract
OBJECTIVES: to investigate the epidemiological traits of carbapenem-non-susceptible Acinetobacter baumannii (CNSAB) and the usefulness of phylogenetic grouping based on partial rpoB gene sequencing in defining the epidemiological traits of CNSAB. METHODS: a total of 547 non-duplicate clinical isolates of Acinetobacter spp. were collected from 19 hospitals in Korea in 2008. Detection of genes encoding OXA carbapenemases and metallo-β-lactamases was performed by PCR. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and repetitive-sequence-based PCR. The 450 bp sequence (zone 2) of the rpoB gene was amplified and sequenced. RESULTS: molecular characterization of the 272 CNSAB isolates identified five sequence types (STs): ST92, ST75, ST137, ST138 and ST69. The first four of these STs were clustered into clonal complex (CC) 92, sharing alleles at six of seven housekeeping gene loci; ST69 shared alleles at five of seven loci. CNSAB of CC92 carried the bla(OXA-23) gene (n = 169), the bla(OXA-51)-like gene preceded by ISAba1 (n = 89) or both (n = 14). Notably, all CNSAB isolates carried a G428T substitution in zone 2 of the rpoB gene. CONCLUSIONS: CNSAB isolates of CC92 with the G428T substitution in zone 2 of the rpoB gene are disseminated nationwide in Korea. A. baumannii with the single nucleotide substitution may be more likely to acquire carbapenem resistance than are other isolates.
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DOI
10.1093/jac/dkq402
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Bae, Il Kwon(배일권)
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Lee, Yang Soon(이양순)
Lee, Jong Han(이종한)
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/92561
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