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Gamma linolenic acid exerts anti-inflammatory and anti-fibrotic effects in diabetic nephropathy

 Do-Hee Kim  ;  Tae-Hyun Yoo  ;  Soon Ha Lee  ;  Hye Young Kang  ;  Bo Young Nam  ;  Seung Jae Kwak  ;  Jwa-Kyung Kim  ;  Jung Tak Park  ;  Seung Hyeok Han  ;  Shin-Wook Kang 
 YONSEI MEDICAL JOURNAL, Vol.53(6) : 1165-1175, 2012 
Journal Title
Issue Date
Animals ; Anti-Inflammatory Agents/therapeutic use* ; Blotting, Western ; Chemokine CCL2/genetics ; Chemokine CCL2/metabolism ; Diabetic Nephropathies/drug therapy* ; Diabetic Nephropathies/metabolism* ; Enzyme-Linked Immunosorbent Assay ; Fibronectins/genetics ; Fibronectins/metabolism ; Intercellular Adhesion Molecule-1/genetics ; Intercellular Adhesion Molecule-1/metabolism ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; alpha-Linolenic Acid/therapeutic use*
Gamma linolenic acid ; experimental diabetic nephropathy ; anti-inflammatory ; anti-fibrotic
PURPOSE: This study was undertaken to investigate the effects of gamma linolenic acid (GLA) on inflammation and extracellular matrix (ECM) synthesis in mesangial and tubular epithelial cells under diabetic conditions. MATERIALS AND METHODS: Sprague-Dawley rats were intraperitoneally injected with either a diluent [n=16, control (C)] or streptozotocin [n=16, diabetes (DM)], and eight rats each from the control and diabetic groups were treated with evening primrose oil by gavage for three months. Rat mesangial cells and NRK-52E cells were exposed to medium containing 5.6 mM glucose and 30 mM glucose (HG), with or without GLA (10 or 100 μM). Intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1), and fibronectin (FN) mRNA and protein expression levels were evaluated. RESULTS: Twenty-four-hour urinary albumin excretion was significantly increased in DM compared to C rats, and GLA treatment significantly reduced albuminuria in DM rats. ICAM-1, MCP-1, FN mRNA and protein expression levels were significantly higher in DM than in C kidneys, and these increases were significantly abrogated by GLA treatment. In vitro, GLA significantly inhibited increases in MCP-1 mRNA expression and protein levels under high glucose conditions in HG-stimulated mesangial and tubular epithelial cells (p<0.05, respectively). ICAM-1 and FN expression showed a similar pattern to the expression of MCP-1. CONCLUSION: GLA attenuates not only inflammation by inhibiting enhanced MCP-1 and ICAM-1 expression, but also ECM accumulation in diabetic nephropathy.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Kang, Hye Young(강혜영)
Kwak, Seung Jae(곽승재)
Kim, Do Hee(김도희)
Kim, Jwa Kyung(김좌경)
Nam, Bo Young(남보영)
Park, Jung Tak(박정탁) ORCID logo https://orcid.org/0000-0002-2325-8982
Yoo, Tae Hyun(유태현) ORCID logo https://orcid.org/0000-0002-9183-4507
Lee, Sun Ha(이순하)
Han, Seung Hyeok(한승혁) ORCID logo https://orcid.org/0000-0001-7923-5635
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