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Hybrid cellular spheroids from hepatocellular carcinoma and insulin-secreting cell lines

Authors
 J.Y. Kim  ;  H.W. Kim  ;  S.J. Bae  ;  D.J. Joo  ;  K.H. Huh  ;  Y.H. Fang  ;  Y. Cho  ;  J.H. Jeong  ;  Y.S. Kim  ;  J.I. Lee 
Citation
 TRANSPLANTATION PROCEEDINGS, Vol.44(4) : 1095-1098, 2012 
Journal Title
 TRANSPLANTATION PROCEEDINGS 
ISSN
 0041-1345 
Issue Date
2012
MeSH
Albumins/metabolism ; Animals ; Apoptosis ; Carcinoma, Hepatocellular/metabolism* ; Carcinoma, Hepatocellular/pathology ; Cell Communication* ; Coculture Techniques ; Hep G2 Cells ; Humans ; Insulin/secretion* ; Insulin-Secreting Cells/pathology ; Insulin-Secreting Cells/secretion* ; Liver Neoplasms/metabolism* ; Liver Neoplasms/pathology ; Rats ; Spheroids, Cellular ; Time Factors
Keywords
Albumins/metabolism ; Animals ; Apoptosis ; Carcinoma, Hepatocellular/metabolism* ; Carcinoma, Hepatocellular/pathology ; Cell Communication* ; Coculture Techniques ; Hep G2 Cells ; Humans ; Insulin/secretion* ; Insulin-Secreting Cells/pathology ; Insulin-Secreting Cells/secretion* ; Liver Neoplasms/metabolism* ; Liver Neoplasms/pathology ; Rats ; Spheroids, Cellular ; Time Factors
Abstract
During islet transplantation into the portal vein of the liver, the islet cells are expected to have complex interactions with hepatocytes. However, the mechanism underlying this interaction is not yet understood. Hence, we developed cellular complexes containing a mixture of human hepatocellular carcinoma cell line (Hep-G2) and rat insulin-secreting cell line (RIN-5F) by using a co-culture model and studied the function and morphology of the resultant hybrid cellular spheroids (HCSs). The RIN-5F and Hep-G2 cells were suspension cultured and, within 5 days of culture, the two types of cells aggregated to yield spheroids. The functionality of the thus formed HCSs was evaluated by measuring the levels of insulin and albumin in the culture supernatant. The HCSs retained their insulin- and albumin-secreting ability and their morphology, as revealed by immunohistological staining. The insulin and albumin levels secreted by the HCSs were considerably higher than those secreted by spheroids of single-cell origin. Generally, obtaining complexes from more than two types of cells is difficult. However, we were able to generate HCSs. We believe that this culture method could have various applications such as studying the in vitro cell-cell interactions and developing new cell transplantation models.
Full Text
http://www.sciencedirect.com/science/article/pii/S0041134512002047
DOI
22564634
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Joon Ye(김준예)
Fang, Yu Hui(방우휘)
Jeong, Jin Ho(정진호)
Cho, Yu Ri(조유리)
Joo, Dong Jin(주동진) ORCID logo https://orcid.org/0000-0001-8405-1531
Huh, Kyu Ha(허규하) ORCID logo https://orcid.org/0000-0003-1364-6989
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91970
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