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Hybrid cellular spheroids from hepatocellular carcinoma and insulin-secreting cell lines

DC Field Value Language
dc.contributor.author김유선-
dc.contributor.author김준예-
dc.contributor.author방우휘-
dc.contributor.author정진호-
dc.contributor.author조유리-
dc.contributor.author주동진-
dc.contributor.author허규하-
dc.date.accessioned2014-12-19T17:44:33Z-
dc.date.available2014-12-19T17:44:33Z-
dc.date.issued2012-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91970-
dc.description.abstractDuring islet transplantation into the portal vein of the liver, the islet cells are expected to have complex interactions with hepatocytes. However, the mechanism underlying this interaction is not yet understood. Hence, we developed cellular complexes containing a mixture of human hepatocellular carcinoma cell line (Hep-G2) and rat insulin-secreting cell line (RIN-5F) by using a co-culture model and studied the function and morphology of the resultant hybrid cellular spheroids (HCSs). The RIN-5F and Hep-G2 cells were suspension cultured and, within 5 days of culture, the two types of cells aggregated to yield spheroids. The functionality of the thus formed HCSs was evaluated by measuring the levels of insulin and albumin in the culture supernatant. The HCSs retained their insulin- and albumin-secreting ability and their morphology, as revealed by immunohistological staining. The insulin and albumin levels secreted by the HCSs were considerably higher than those secreted by spheroids of single-cell origin. Generally, obtaining complexes from more than two types of cells is difficult. However, we were able to generate HCSs. We believe that this culture method could have various applications such as studying the in vitro cell-cell interactions and developing new cell transplantation models.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAlbumins/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis-
dc.subject.MESHCarcinoma, Hepatocellular/metabolism*-
dc.subject.MESHCarcinoma, Hepatocellular/pathology-
dc.subject.MESHCell Communication*-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHHep G2 Cells-
dc.subject.MESHHumans-
dc.subject.MESHInsulin/secretion*-
dc.subject.MESHInsulin-Secreting Cells/pathology-
dc.subject.MESHInsulin-Secreting Cells/secretion*-
dc.subject.MESHLiver Neoplasms/metabolism*-
dc.subject.MESHLiver Neoplasms/pathology-
dc.subject.MESHRats-
dc.subject.MESHSpheroids, Cellular-
dc.subject.MESHTime Factors-
dc.titleHybrid cellular spheroids from hepatocellular carcinoma and insulin-secreting cell lines-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorJ.Y. Kim-
dc.contributor.googleauthorH.W. Kim-
dc.contributor.googleauthorS.J. Bae-
dc.contributor.googleauthorD.J. Joo-
dc.contributor.googleauthorK.H. Huh-
dc.contributor.googleauthorY.H. Fang-
dc.contributor.googleauthorY. Cho-
dc.contributor.googleauthorJ.H. Jeong-
dc.contributor.googleauthorY.S. Kim-
dc.contributor.googleauthorJ.I. Lee-
dc.identifier.doi22564634-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00785-
dc.contributor.localIdA00956-
dc.contributor.localIdA01789-
dc.contributor.localIdA03749-
dc.contributor.localIdA03872-
dc.contributor.localIdA03948-
dc.contributor.localIdA04344-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid22564634-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0041134512002047-
dc.subject.keywordAlbumins/metabolism-
dc.subject.keywordAnimals-
dc.subject.keywordApoptosis-
dc.subject.keywordCarcinoma, Hepatocellular/metabolism*-
dc.subject.keywordCarcinoma, Hepatocellular/pathology-
dc.subject.keywordCell Communication*-
dc.subject.keywordCoculture Techniques-
dc.subject.keywordHep G2 Cells-
dc.subject.keywordHumans-
dc.subject.keywordInsulin/secretion*-
dc.subject.keywordInsulin-Secreting Cells/pathology-
dc.subject.keywordInsulin-Secreting Cells/secretion*-
dc.subject.keywordLiver Neoplasms/metabolism*-
dc.subject.keywordLiver Neoplasms/pathology-
dc.subject.keywordRats-
dc.subject.keywordSpheroids, Cellular-
dc.subject.keywordTime Factors-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameKim, Joon Ye-
dc.contributor.alternativeNameFang, Yu Hui-
dc.contributor.alternativeNameJeong, Jin Ho-
dc.contributor.alternativeNameCho, Yu Ri-
dc.contributor.alternativeNameJoo, Dong Jin-
dc.contributor.alternativeNameHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorKim, Joon Ye-
dc.contributor.affiliatedAuthorFang, Yu Hui-
dc.contributor.affiliatedAuthorJeong, Jin Ho-
dc.contributor.affiliatedAuthorCho, Yu Ri-
dc.contributor.affiliatedAuthorJoo, Dong Jin-
dc.contributor.affiliatedAuthorHuh, Kyu Ha-
dc.citation.volume44-
dc.citation.number4-
dc.citation.startPage1095-
dc.citation.endPage1098-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.44(4) : 1095-1098, 2012-
dc.identifier.rimsid30038-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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