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Characterization of different subpopulations from bone marrow-derived mesenchymal stromal cells by alkaline phosphatase expression

Authors
 Yun Hee Kim  ;  Dong Suk Yoon  ;  Hyun Ok Kim  ;  Jin Woo Lee 
Citation
 Stem Cells and Development, Vol.21(16) : 2956-2968, 2012 
Journal Title
 Stem Cells and Development 
ISSN
 1547-3287 
Issue Date
2012
MeSH
Adipocytes/cytology ; Adipogenesis/genetics ; Adult ; Aged ; Alkaline Phosphatase/metabolism* ; Biomarkers/metabolism ; Cell Cycle/genetics ; Cell Lineage/genetics ; Cell Proliferation ; Cell Size ; Chondrogenesis/genetics ; Clone Cells ; Colony-Forming Units Assay ; Female ; Fetal Blood/cytology ; Flow Cytometry ; Gene Expression Regulation ; Humans ; Male ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/enzymology* ; Middle Aged ; Osteoblasts/cytology ; Osteogenesis/genetics ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Tissue Donors ; Young Adult
Keywords
Adipocytes/cytology ; Adipogenesis/genetics ; Adult ; Aged ; Alkaline Phosphatase/metabolism* ; Biomarkers/metabolism ; Cell Cycle/genetics ; Cell Lineage/genetics ; Cell Proliferation ; Cell Size ; Chondrogenesis/genetics ; Clone Cells ; Colony-Forming Units Assay ; Female ; Fetal Blood/cytology ; Flow Cytometry ; Gene Expression Regulation ; Humans ; Male ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/enzymology* ; Middle Aged ; Osteoblasts/cytology ; Osteogenesis/genetics ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Tissue Donors ; Young Adult
Abstract
Multiple surface markers have been utilized for the enrichment of bone marrow mesenchymal stromal cells (MSCs) and to define primitive stem cells. We classified human bone marrow-derived MSC populations according to tissue nonspecific alkaline phosphatase (TNAP) activity. TNAP expression varied among unexpanded primary MSCs, and its level was not related to colony-forming activity or putative surface markers, such as CD105 and CD29, donor age, or gender. TNAP levels were increased in larger cells, and a colony-forming unit-fibroblast assay revealed that the colony size was decreased during in vitro expansion. TNAP-positive (TNAP+) MSCs showed limited multipotential capacity, whereas TNAP-negative (TNAP-) MSCs retained the differentiation potential into 3 lineages (osteogenic-, adipogenic-, and chondrogenic differentiation). High degree of calcium mineralization and high level of osteogenic-related gene expression (osteopontin, dlx5, and cbfa1) were found in TNAP+ cells. In contrast, during chondrogenic differentiation, type II collagen was successfully induced in TNAP- cells, but not in TNAP+ cells. TNAP+ cells showed high levels of the hypertrophic markers, type X collagen and cbfa1. Mesenchymal stem cell antigen-1 (MSCA-1) is identical to TNAP. Therefore, TNAP+ cells were sorted by using antibody targeting MSCA-1. MSCA-1-positive cells sorted for TNAP+ cells exhibited low proliferation rates. Expression of cell cycle-related genes (cyclin A2, CDK2, and CDK4) and pluripotency marker genes (rex1 and nanog) were higher in TNAP- MSC than in TNAP+ MSC. Therefore, TNAP- cells can be described as more primitive bone marrow-derived cells and TNAP levels in MSCs can be used to predict chondrocyte hypertrophy or osteogenic capacity.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/scd.2011.0349
DOI
22702738
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Yoon, Dong Suk(윤동석)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91868
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