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Characterization of different subpopulations from bone marrow-derived mesenchymal stromal cells by alkaline phosphatase expression

DC FieldValueLanguage
dc.contributor.author김현옥-
dc.contributor.author윤동석-
dc.contributor.author이진우-
dc.date.accessioned2014-12-19T17:41:23Z-
dc.date.available2014-12-19T17:41:23Z-
dc.date.issued2012-
dc.identifier.issn1547-3287-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91868-
dc.description.abstractMultiple surface markers have been utilized for the enrichment of bone marrow mesenchymal stromal cells (MSCs) and to define primitive stem cells. We classified human bone marrow-derived MSC populations according to tissue nonspecific alkaline phosphatase (TNAP) activity. TNAP expression varied among unexpanded primary MSCs, and its level was not related to colony-forming activity or putative surface markers, such as CD105 and CD29, donor age, or gender. TNAP levels were increased in larger cells, and a colony-forming unit-fibroblast assay revealed that the colony size was decreased during in vitro expansion. TNAP-positive (TNAP+) MSCs showed limited multipotential capacity, whereas TNAP-negative (TNAP-) MSCs retained the differentiation potential into 3 lineages (osteogenic-, adipogenic-, and chondrogenic differentiation). High degree of calcium mineralization and high level of osteogenic-related gene expression (osteopontin, dlx5, and cbfa1) were found in TNAP+ cells. In contrast, during chondrogenic differentiation, type II collagen was successfully induced in TNAP- cells, but not in TNAP+ cells. TNAP+ cells showed high levels of the hypertrophic markers, type X collagen and cbfa1. Mesenchymal stem cell antigen-1 (MSCA-1) is identical to TNAP. Therefore, TNAP+ cells were sorted by using antibody targeting MSCA-1. MSCA-1-positive cells sorted for TNAP+ cells exhibited low proliferation rates. Expression of cell cycle-related genes (cyclin A2, CDK2, and CDK4) and pluripotency marker genes (rex1 and nanog) were higher in TNAP- MSC than in TNAP+ MSC. Therefore, TNAP- cells can be described as more primitive bone marrow-derived cells and TNAP levels in MSCs can be used to predict chondrocyte hypertrophy or osteogenic capacity.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfStem Cells and Development-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleCharacterization of different subpopulations from bone marrow-derived mesenchymal stromal cells by alkaline phosphatase expression-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학)-
dc.contributor.googleauthorYun Hee Kim-
dc.contributor.googleauthorDong Suk Yoon-
dc.contributor.googleauthorHyun Ok Kim-
dc.contributor.googleauthorJin Woo Lee-
dc.identifier.doi10.1089/scd.2011.0349-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01122-
dc.contributor.localIdA02547-
dc.contributor.localIdA03230-
dc.relation.journalcodeJ02684-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/scd.2011.0349-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.alternativeNameYoon, Dong Suk-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.contributor.affiliatedAuthorYoon, Dong Suk-
dc.contributor.affiliatedAuthorLee, Jin Woo-
dc.citation.volume21-
dc.citation.number16-
dc.citation.startPage2956-
dc.citation.endPage2968-
dc.identifier.bibliographicCitationStem Cells and Development, Vol.21(16) : 2956-2968, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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