203 288

Cited 0 times in

A novel multiplex real-time PCR assay for the concurrent detection of hepatitis A, B and C viruses in patients with acute hepatitis

Authors
 Yongjung Park  ;  Beom Seok Kim  ;  Kyu Hun Choi  ;  Dong Ho Shin  ;  Mi Jung Lee  ;  Yonggeun Cho  ;  Hyon- Suk Kim 
Citation
 PLOS ONE, Vol.7(11) : e49106, 2012 
Journal Title
 PLOS ONE 
Issue Date
2012
MeSH
Adolescent ; Adult ; Female ; Hepacivirus/genetics ; Hepacivirus/isolation & purification* ; Hepatitis/diagnosis ; Hepatitis/virology* ; Hepatitis A virus/genetics ; Hepatitis A virus/isolation & purification* ; Hepatitis B virus/genetics ; Hepatitis B virus/isolation & purification* ; Humans ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Viral Load
Keywords
Adolescent ; Adult ; Female ; Hepacivirus/genetics ; Hepacivirus/isolation & purification* ; Hepatitis/diagnosis ; Hepatitis/virology* ; Hepatitis A virus/genetics ; Hepatitis A virus/isolation & purification* ; Hepatitis B virus/genetics ; Hepatitis B virus/isolation & purification* ; Humans ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Viral Load
Abstract
A novel multiplex real-time PCR assay for concurrent detection of hepatitis viruses was evaluated for its clinical performance in screening patients with acute hepatitis. A total of 648 serum samples were collected from patients with acute symptoms of hepatitis. Concurrent detection of nucleic acids of HAV, HBV and HCV was performed using the Magicplex™ HepaTrio Real-time Detection test. Serum nucleic acid levels of HBV and HCV were also quantified by the Cobas® AmpliPrep/Cobas® TaqMan® (CAP/CTM) HBV and HCV tests. Patients' medical records were also reviewed. Concordance rates between the results from the HepaTrio and the CAP/CTM tests for the detection of HBV and HCV were 94.9% (k = 0.88) and 99.2% (k = 0.98), respectively. The cycle threshold values with the HepaTrio test were also correlated well with the levels of HBV DNA (r = -0.9230) and HCV RNA (r = -0.8458). The sensitivity and specificity of the HepaTrio test were 93.8% and 98.2%, respectively, for detecting HBV infection, and 99.1% and 100.0%, respectively, for HCV infection. For the HepaTrio test, 21 (3.2%) cases were positive for both HBV and HCV. Among the positive cases, 6 (0.9%) were true coinfections. This test also detected 18 (2.8%) HAV positives. The HepaTrio test demonstrated good clinical performance and produced results that agreed well with those of the CAP/CTM assays, especially for the detection of HCV. This assay was also able to detect HAV RNA from anti-HAV IgM-positive individuals. Therefore, this new multiplex PCR assay could be useful for the concurrent detection of the three hepatitis viruses.
Files in This Item:
T201203954.pdf Download
DOI
23145085
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Beom Seok(김범석) ORCID logo https://orcid.org/0000-0002-5732-2583
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정)
Lee, Mi Jung(이미정)
Cho, Yonggeun(조용근) ORCID logo https://orcid.org/0000-0003-1946-4318
Choi, Kyu Hun(최규헌) ORCID logo https://orcid.org/0000-0003-0095-9011
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91723
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse