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Identification and characterization of a noncoding RNA at the mouse Pcna locus.

Authors
 Ji-Yeon Lee  ;  Abdul Aziz Khan  ;  Hyehyun Min  ;  Xinnan Wang  ;  Myoung Hee Kim 
Citation
 MOLECULES AND CELLS, Vol.33(2) : 111-116, 2012 
Journal Title
 MOLECULES AND CELLS 
ISSN
 1016-8478 
Issue Date
2012
MeSH
Animals ; Base Sequence ; Carcinoma, Lewis Lung ; Fibroblasts/metabolism ; Gene Expression Regulation, Neoplastic* ; Genetic Loci/genetics ; Melanoma, Experimental ; Mice ; Molecular Sequence Data ; NIH 3T3 Cells ; Proliferating Cell Nuclear Antigen/genetics* ; Promoter Regions, Genetic/genetics ; RNA, Small Interfering/genetics ; RNA, Untranslated/genetics ; RNA, Untranslated/metabolism* ; Regulatory Sequences, Nucleic Acid/genetics
Keywords
bidirectional promoter ; co-expression ; gene regulation ; non-coding RNA ; Pcna
Abstract
AK007836 encodes a noncoding RNA (ncRNA) consisting of 2 exons. Since AK007836 is located just upstream of Pcna and transcribed in the opposite direction to that of Pcna, we analyzed its expression pattern. Both ncRNA and Pcna expressions were detected in in vitro and in vivo cells, showing a positive correlation. A 177 bp region separating the first exons of Pcna and AK007836 has a bidirectional promoter activity. When the expression of ncRNA was reduced by siRNA, Pcna expression was also reduced in normal cells, but not in cancer cells. These results suggest that the ncRNA is divergently transcribed from the bidirectional promoter, positively regulating the neighboring protein-coding Pcna gene transcription, and this regulatory function is somehow disrupted in cancer cells.
Full Text
http://link.springer.com/article/10.1007%2Fs10059-012-2164-x
DOI
22228179
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Min, Hye Hyun(민혜현) ORCID logo https://orcid.org/0000-0001-5450-5088
Lee, Ji Yeon(이지연) ORCID logo https://orcid.org/0000-0002-0670-3095
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91483
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