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Proteomic analysis of microvesicles derived from human mesenchymal stem cells

 Han-Soo Kim  ;  Do-Young Choi  ;  So Jeong Yun  ;  Seong-Mi Choi  ;  Jeong Won Kang  ;  Jin Woo Jung  ;  Daehee Hwang  ;  Kwang Pyo Kim  ;  Dong-Wook Kim 
 Journal of Proteome Research, Vol.11(2) : 839-849, 2012 
Journal Title
 Journal of Proteome Research 
Issue Date
Cell Differentiation/physiology ; Cell Growth Processes/physiology ; Cells, Cultured ; Cytoplasmic Vesicles/chemistry* ; Cytoplasmic Vesicles/metabolism ; Drug Discovery ; Humans ; Mesenchymal Stromal Cells/chemistry ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/metabolism ; Proteins/analysis* ; Proteins/chemistry ; Proteins/classification ; Proteome/analysis ; Proteomics/methods* ; Signal Transduction
mesenchymal stem cells ; microvesicle ; proteomics ; self-renewal ; tissue regeneration
Mesenchymal stem cells (MSCs) have emerged as a promising means for treating degenerative or incurable diseases. Recent studies have shown that microvesicles (MVs) from MSCs (MSC-MVs) contribute to recovery of damaged tissues in animal disease models. Here, we profiled the MSC-MV proteome to investigate their therapeutic effects. LC-MS/MS analysis of MSC-MVs identified 730 MV proteins. The MSC-MV proteome included five positive and two variable known markers of MSCs, but no negative marker, as well as 43 surface receptors and signaling molecules controlling self-renewal and differentiation of MSCs. Functional enrichment analysis showed that cellular processes represented by the MSC-MV proteins include cell proliferation, adhesion, migration, and morphogenesis. Integration of MSC's self-renewal and differentiation-related genes and the proteome of MSC-conditioned media (MSC-CM) with the MSC-MV proteome revealed potential MV protein candidates that can be associated with the therapeutic effects of MSC-MVs: (1) surface receptors (PDGFRB, EGFR, and PLAUR); (2) signaling molecules (RRAS/NRAS, MAPK1, GNA13/GNG12, CDC42, and VAV2); (3) cell adhesion (FN1, EZR, IQGAP1, CD47, integrins, and LGALS1/LGALS3); and (4) MSC-associated antigens (CD9, CD63, CD81, CD109, CD151, CD248, and CD276). Therefore, the MSC-MV proteome provides a comprehensive basis for understanding the potential of MSC-MVs to affect tissue repair and regeneration.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
Kim, Han Soo(김한수)
Choi, Seong Mi(최성미)
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