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Gustatory receptors required for avoiding the insecticide L-canavanine

Authors
 Youngseok Lee  ;  Min Jung Kang  ;  Jaewon Shim  ;  Chae Uk Cheong  ;  Seok Jun Moon  ;  Craig Montell 
Citation
 JOURNAL OF NEUROSCIENCE, Vol.32(4) : 1429-1435, 2012 
Journal Title
 JOURNAL OF NEUROSCIENCE 
ISSN
 0270-6474 
Issue Date
2012
MeSH
Action Potentials/genetics ; Action Potentials/physiology ; Animals ; Animals, Genetically Modified ; Avoidance Learning/drug effects ; Avoidance Learning/physiology* ; Canavanine/pharmacology* ; Drosophila Proteins/genetics ; Drosophila Proteins/physiology* ; Insecticides/pharmacology* ; Mutation ; Protein Subunits/genetics ; Protein Subunits/physiology ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/physiology*
Keywords
Action Potentials/genetics ; Action Potentials/physiology ; Animals ; Animals, Genetically Modified ; Avoidance Learning/drug effects ; Avoidance Learning/physiology* ; Canavanine/pharmacology* ; Drosophila Proteins/genetics ; Drosophila Proteins/physiology* ; Insecticides/pharmacology* ; Mutation ; Protein Subunits/genetics ; Protein Subunits/physiology ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/physiology*
Abstract
Insect survival depends on contact chemosensation to sense and avoid consuming plant-derived insecticides, such as L-canavanine. Members of a family of ∼60 gustatory receptors (GRs) comprise the main peripheral receptors responsible for taste sensation in Drosophila. However, the roles of most Drosophila GRs are unknown. In addition to GRs, a G protein-coupled receptor, DmXR, has been reported to be required for detecting L-canavanine. Here, we showed that GRs are essential for responding to L-canavanine and that flies missing DmXR displayed normal L-canavanine avoidance and L-canavanine-evoked action potentials. Mutations disrupting either Gr8a or Gr66a resulted in an inability to detect L-canavanine. We found that L-canavanine stimulated action potentials in S-type sensilla, which were where Gr8a and Gr66a were both expressed, but not in Gr66a-expressing sensilla that did not express Gr8a. L-canavanine-induced action potentials were also abolished in the Gr8a and Gr66a mutant animals. Gr8a was narrowly required for responding to L-canavanine, in contrast to Gr66a, which was broadly required for responding to other noxious tastants. Our data suggest that GR8a and GR66a are subunits of an L-canavanine receptor and that GR8a contributes to the specificity for L-canavanine.
Files in This Item:
T201200467.pdf Download
DOI
22279227
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kang, Min Jung(강민정)
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Shim, Jae Won(심재원)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90999
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