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Gustatory receptors required for avoiding the insecticide L-canavanine

DC Field Value Language
dc.contributor.author강민정-
dc.contributor.author문석준-
dc.contributor.author심재원-
dc.date.accessioned2014-12-19T17:13:08Z-
dc.date.available2014-12-19T17:13:08Z-
dc.date.issued2012-
dc.identifier.issn0270-6474-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/90999-
dc.description.abstractInsect survival depends on contact chemosensation to sense and avoid consuming plant-derived insecticides, such as L-canavanine. Members of a family of ∼60 gustatory receptors (GRs) comprise the main peripheral receptors responsible for taste sensation in Drosophila. However, the roles of most Drosophila GRs are unknown. In addition to GRs, a G protein-coupled receptor, DmXR, has been reported to be required for detecting L-canavanine. Here, we showed that GRs are essential for responding to L-canavanine and that flies missing DmXR displayed normal L-canavanine avoidance and L-canavanine-evoked action potentials. Mutations disrupting either Gr8a or Gr66a resulted in an inability to detect L-canavanine. We found that L-canavanine stimulated action potentials in S-type sensilla, which were where Gr8a and Gr66a were both expressed, but not in Gr66a-expressing sensilla that did not express Gr8a. L-canavanine-induced action potentials were also abolished in the Gr8a and Gr66a mutant animals. Gr8a was narrowly required for responding to L-canavanine, in contrast to Gr66a, which was broadly required for responding to other noxious tastants. Our data suggest that GR8a and GR66a are subunits of an L-canavanine receptor and that GR8a contributes to the specificity for L-canavanine.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfJOURNAL OF NEUROSCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAction Potentials/genetics-
dc.subject.MESHAction Potentials/physiology-
dc.subject.MESHAnimals-
dc.subject.MESHAnimals, Genetically Modified-
dc.subject.MESHAvoidance Learning/drug effects-
dc.subject.MESHAvoidance Learning/physiology*-
dc.subject.MESHCanavanine/pharmacology*-
dc.subject.MESHDrosophila Proteins/genetics-
dc.subject.MESHDrosophila Proteins/physiology*-
dc.subject.MESHInsecticides/pharmacology*-
dc.subject.MESHMutation-
dc.subject.MESHProtein Subunits/genetics-
dc.subject.MESHProtein Subunits/physiology-
dc.subject.MESHReceptors, Cell Surface/genetics-
dc.subject.MESHReceptors, Cell Surface/physiology*-
dc.titleGustatory receptors required for avoiding the insecticide L-canavanine-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorYoungseok Lee-
dc.contributor.googleauthorMin Jung Kang-
dc.contributor.googleauthorJaewon Shim-
dc.contributor.googleauthorChae Uk Cheong-
dc.contributor.googleauthorSeok Jun Moon-
dc.contributor.googleauthorCraig Montell-
dc.identifier.doi10.1523/JNEUROSCI.4630-11.2012-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00028-
dc.contributor.localIdA01358-
dc.contributor.localIdA02208-
dc.relation.journalcodeJ01633-
dc.identifier.eissn1529-2401-
dc.identifier.pmid22279227-
dc.contributor.alternativeNameKang, Min Jung-
dc.contributor.alternativeNameMoon, Seok Jun-
dc.contributor.alternativeNameShim, Jae Won-
dc.contributor.affiliatedAuthorKang, Min Jung-
dc.contributor.affiliatedAuthorMoon, Seok Jun-
dc.contributor.affiliatedAuthorShim, Jae Won-
dc.citation.volume32-
dc.citation.number4-
dc.citation.startPage1429-
dc.citation.endPage1435-
dc.identifier.bibliographicCitationJOURNAL OF NEUROSCIENCE, Vol.32(4) : 1429-1435, 2012-
dc.identifier.rimsid33267-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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