5 440

Cited 0 times in

Leukotriene B(4) receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products.

 Young Hee Nam  ;  Arim Min  ;  Seong Hoon Kim  ;  Young Ah Lee  ;  Kyeong Ah Kim  ;  Kyoung-Ju Song  ;  Myeong Heon Shin 
 INFLAMMATION RESEARCH, Vol.61(2) : 97-102, 2012 
Journal Title
Issue Date
Benzoquinones/pharmacology ; Endopeptidase K/pharmacology ; Fatty Alcohols/pharmacology ; Glycols/pharmacology ; Hot Temperature ; Humans ; Interleukin-8/biosynthesis ; Interleukin-8/immunology* ; Interleukin-8/secretion ; Leukotriene Antagonists/pharmacology ; Leukotriene B4/analysis ; Lipase/pharmacology ; Lipoxygenase Inhibitors/pharmacology ; Neutrophil Activation/drug effects ; Neutrophil Activation/immunology ; Neutrophils/drug effects ; Neutrophils/immunology* ; Neutrophils/parasitology ; Piperazines/pharmacology ; Receptors, Leukotriene B4/antagonists & inhibitors ; Receptors, Leukotriene B4/immunology* ; Tetrazoles/pharmacology ; Thiazoles/pharmacology ; Trichomonas Infections/immunology* ; Trichomonas Infections/parasitology ; Trichomonas vaginalis/drug effects ; Trichomonas vaginalis/immunology*
Trichomonas vaginalis ; Human neutrophils ; IL-8 ; Leukotriene B4 receptors BLT1 ; BLT2
OBJECTIVE AND METHOD: Trichomonas vaginalis is a flagellated protozoan parasite that causes human trichomoniasis. Although T. vaginalis itself can secrete lipid mediator leukotriene (LT) B(4) leading to neutrophil activation, information regarding the signaling mechanism involved in neutrophil activation induced by T. vaginalis-secreted LTB(4) is limited. We investigated whether LTB(4) contained in the T. vaginalis-derived secretory products (TvSP) is closely involved in interleukin (IL)-8 production in human neutrophils via LTB(4) receptors BLT1 or BLT2.

RESULTS: T. vaginalis produced more than 714 pg/ml of LTB(4) per 1 × 10(7) trichomonads. The ability of trichomonads to secrete LTB(4) was inhibited by treatment of trichomonads with the 5-lipo-oxygenease inhibitor AA861, but not the cyclo-oxygenease I inhibitor FR122047. When neutrophils were incubated with TvSP obtained from 1 × 10(7) trichomonads, IL-8 protein secretion was significantly increased compared to results for cells incubated with medium alone. The stimulatory effect of TvSP on IL-8 production was strongly inhibited by pretreatment of TvSP with lipase, although pretreatment with heat or proteinase K showed little inhibitory effect. Moreover, TvSP-induced IL-8 production was efficiently inhibited when trichomonads were pretreated with AA861 or when neutrophils were pretreated with antagonists for BLT1 or BLT2.

CONCLUSION: Our results suggest that LTB(4) receptors BLT1 and BLT2 are involved in IL-8 production in neutrophils induced by T. vaginalis.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Ah(김경아)
Nam, Young Hee(남영희)
Min, A Rim(민아림) ORCID logo https://orcid.org/0000-0003-2938-9630
Song, Kyoung Ju(송경주)
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.