4 233

Cited 0 times in

Differential expression patterns of MMPs and their role in the invasion of epithelial premalignant tumors and invasive cutaneous squamous cell carcinoma.

Authors
 Mi Ryung Roha  ;  Zhenlong Zhenga  ;  Hyun Sook Kim  ;  Ji Eun Kwon  ;  Hei-Cheul Jeung  ;  Sun Young Rha  ;  Kee Yang Chung 
Citation
 EXPERIMENTAL AND MOLECULAR PATHOLOGY, Vol.92(2) : 236-242, 2012 
Journal Title
 EXPERIMENTAL AND MOLECULAR PATHOLOGY 
ISSN
 0014-4800 
Issue Date
2012
MeSH
Bowen's Disease/enzymology ; Bowen's Disease/pathology ; Carcinoma, Squamous Cell/enzymology ; Carcinoma, Squamous Cell/pathology* ; Cell Line, Tumor ; Humans ; Matrix Metalloproteinases/biosynthesis* ; Neoplasm Invasiveness/pathology ; Precancerous Conditions/enzymology ; Precancerous Conditions/pathology ; Skin/enzymology ; Skin/pathology ; Skin Neoplasms/enzymology ; Skin Neoplasms/pathology*
Keywords
Matrix metalloproteinase ; Cutaneous squamous cell carcinoma ; Tumor invasion
Abstract
Co-expression of several members of the matrix metalloproteinase (MMP) family is characteristic of human malignant tumors. MMP-2, MMP-9, TIMP-2, and MT1-MMP are thought to be involved in the process of destruction of basement membranes and stromal invasion by neoplastic epithelial cells. In this study, we investigated the expression and role of MMPs in cutaneous oncogenesis. Tissue microarray consisting of 62 squamous cell carcinomas (SCC), 32 Bowen's disease (BD) samples, 25 normal epidermis samples were obtained for the study. MMP-2,-9, MT1-MMP and TIMP-2 proteins were examined by immunohistochemical staining and mRNA level was detected by quantitative RT-PCR in fresh tissues consisting of 5 cutaneous SCCs and paired normal epidermis samples. Gelatinase activity of MMP-2 and MMP-9 was investigated by gelatin zymography and protein levels of MT1-MMP and TIMP-2 were measured by western blot in 2 human SCC cell lines. The invasive property was evaluated with invasion assays using Transwell filters. SCC exhibited significantly increased MMP-2, MT1-MMP and decreased TIMP-2 mRNA and protein expression compared to that of the normal epithelium. Immunohistochemical staining revealed that MT1-MMP was strongly expressed on the invasive front of SCCs, whereas BD exhibited higher expression around the dyskeratotic cells in the epithelium. In comparison with the expression observed in BD, SCC exhibited significantly increased MMP-2 expression. In addition, high MMP-2 and MT1-MMP expression and low TIMP-2 expression had a significant positive correlation with the invasiveness of SCC cell lines in vitro. Our results revealed significantly increased MT1-MMP and MMP-2 expression and decreased TIMP-2 expression in cutaneous SCC, and the expression correlated with the invasiveness of SCC cell lines. Therefore, the expression of these factors in cutaneous tumors may serve as an indicator of tumor aggressiveness and invasion.
Full Text
http://www.sciencedirect.com/science/article/pii/S001448001200010X
DOI
22305927
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Roh, Mi Ryung(노미령) ORCID logo https://orcid.org/0000-0002-6285-2490
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Chung, Kee Yang(정기양) ORCID logo https://orcid.org/0000-0003-3257-0297
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90269
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse