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Comparison of automated multiplexed bead-based ANA screening assay with ELISA for detecting five common anti-extractable nuclear antigens and anti-dsDNA in systemic rheumatic diseases.

Authors
 Yoonjung Kim  ;  Yongjung Park  ;  Eun Young Lee  ;  Hyon-Suk Kim 
Citation
 CLINICA CHIMICA ACTA, Vol.413(1-2) : 308-311, 2012 
Journal Title
 CLINICA CHIMICA ACTA 
ISSN
 0009-8981 
Issue Date
2012
MeSH
Antigens, Nuclear/analysis* ; Automation* ; DNA/immunology* ; Enzyme-Linked Immunosorbent Assay/methods* ; Humans ; Microscopy, Fluorescence ; Rheumatic Diseases/immunology*
Keywords
Multiplex bead-based assay ; BioPlex™ 2200 system ; Autoantibodies ; Systemic lupus erythematosus ; Sjögren syndrome
Abstract
BACKGROUND: A newly developed and totally automated Luminex-based assay, the BioPlex™ 2200 system, is able to detect various autoantibodies simultaneously from a single sample. We compared the BioPlex™ 2200 system with ELISA for the detection of six autoantibodies. METHODS: A total of 127 serum samples from the patients with systemic rheumatic diseases were collected and assayed with the BioPlex™ 2200 system (Bio-Rad, USA) and conventional ELISA (INOVA Diagnostics, USA) for 5 anti-extractable nuclear antigens. Additionally, relative sensitivity of the BioPlex™ 2200 system for detecting anti-dsDNA was evaluated with 79 specimens from SLE patients, which were positive for anti-dsDNA by ELISA. RESULTS: The concordance rates between ELISA and the BioPlex ranged from 88.1% for anti-RNP to 95.2% for anti-Scl-70, and the kappa coefficients between the results by the two assays were from 0.48 to 0.67. Among the 79 anti-dsDNA positive specimens by ELISA, seventy-eight (98.7%) showed positive results for anti-dsDNA by the BioPlex. CONCLUSIONS: The BioPlex™ 2200 system showed comparable results with those by conventional ELISA for detecting autoantibodies, and this automated assay could measure multifarious autoantibodies concurrently in a single sample. It could be effectively used in clinical laboratories for screening autoimmune diseases.
Full Text
http://www.sciencedirect.com/science/article/pii/S000989811100578X
DOI
22027339
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89971
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