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Comparison of automated multiplexed bead-based ANA screening assay with ELISA for detecting five common anti-extractable nuclear antigens and anti-dsDNA in systemic rheumatic diseases.
DC Field | Value | Language |
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dc.contributor.author | 김현숙 | - |
dc.contributor.author | 박용정 | - |
dc.date.accessioned | 2014-12-19T16:40:21Z | - |
dc.date.available | 2014-12-19T16:40:21Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0009-8981 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89971 | - |
dc.description.abstract | BACKGROUND: A newly developed and totally automated Luminex-based assay, the BioPlex™ 2200 system, is able to detect various autoantibodies simultaneously from a single sample. We compared the BioPlex™ 2200 system with ELISA for the detection of six autoantibodies. METHODS: A total of 127 serum samples from the patients with systemic rheumatic diseases were collected and assayed with the BioPlex™ 2200 system (Bio-Rad, USA) and conventional ELISA (INOVA Diagnostics, USA) for 5 anti-extractable nuclear antigens. Additionally, relative sensitivity of the BioPlex™ 2200 system for detecting anti-dsDNA was evaluated with 79 specimens from SLE patients, which were positive for anti-dsDNA by ELISA. RESULTS: The concordance rates between ELISA and the BioPlex ranged from 88.1% for anti-RNP to 95.2% for anti-Scl-70, and the kappa coefficients between the results by the two assays were from 0.48 to 0.67. Among the 79 anti-dsDNA positive specimens by ELISA, seventy-eight (98.7%) showed positive results for anti-dsDNA by the BioPlex. CONCLUSIONS: The BioPlex™ 2200 system showed comparable results with those by conventional ELISA for detecting autoantibodies, and this automated assay could measure multifarious autoantibodies concurrently in a single sample. It could be effectively used in clinical laboratories for screening autoimmune diseases. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | CLINICA CHIMICA ACTA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antigens, Nuclear/analysis* | - |
dc.subject.MESH | Automation* | - |
dc.subject.MESH | DNA/immunology* | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microscopy, Fluorescence | - |
dc.subject.MESH | Rheumatic Diseases/immunology* | - |
dc.title | Comparison of automated multiplexed bead-based ANA screening assay with ELISA for detecting five common anti-extractable nuclear antigens and anti-dsDNA in systemic rheumatic diseases. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Yoonjung Kim | - |
dc.contributor.googleauthor | Yongjung Park | - |
dc.contributor.googleauthor | Eun Young Lee | - |
dc.contributor.googleauthor | Hyon-Suk Kim | - |
dc.identifier.doi | 22027339 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01582 | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J00543 | - |
dc.identifier.eissn | 1873-3492 | - |
dc.identifier.pmid | 22027339 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S000989811100578X | - |
dc.subject.keyword | Multiplex bead-based assay | - |
dc.subject.keyword | BioPlex™ 2200 system | - |
dc.subject.keyword | Autoantibodies | - |
dc.subject.keyword | Systemic lupus erythematosus | - |
dc.subject.keyword | Sjögren syndrome | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.alternativeName | Park, Yong Jung | - |
dc.contributor.affiliatedAuthor | Park, Yong Jung | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.citation.volume | 413 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 308 | - |
dc.citation.endPage | 311 | - |
dc.identifier.bibliographicCitation | CLINICA CHIMICA ACTA, Vol.413(1-2) : 308-311, 2012 | - |
dc.identifier.rimsid | 32010 | - |
dc.type.rims | ART | - |
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