Cited 0 times in

63 2

Host cell autophagy activated by antibiotics is required for their effective antimycobacterial drug action.

Authors
 Jwa-Jin Kim ; Hye-Mi Lee ; Tamotsu Yoshimori ; JongKyeong Chung ; Jin Bong Park ; Young Kil Park ; Heekyung Yoo ; Sung Jae Shin ; Eun-Kyeong Jo ; Zee-Won Lee ; Jin-Man Kim ; Guang-Ho Cha ; Sang-Hee Lee ; Hyo Sun Jin ; Jae-Min Yuk ; Wonho Kim ; Dong-Min Shin 
Citation
 Cell Host & Microbe, Vol.11(5) : 457~468, 2012 
Journal Title
 Cell Host & Microbe 
ISSN
 1931-3128 
Issue Date
2012
Abstract
The current standard of treatment against tuberculosis consists of a cocktail of first-line drugs, including isoniazid and pyrazinamide. Although these drugs are known to be bactericidal, contribution of host cell responses in the context of antimycobacterial chemotherapy, if any, remains unknown. We demonstrate that isoniazid and pyrazinamide promote autophagy activation and phagosomal maturation in Mycobacterium tuberculosis (Mtb)-infected host cells. Treatment of Mtb-infected macrophages with isoniazid or pyrazinamide caused significant activation of cellular and mitochondrial reactive oxygen species and autophagy, which was triggered by bacterial hydroxyl radical generation. Mycobacterium marinum-infected autophagy-defective, atg7 mutant Drosophila exhibited decreased survival rates, which could not be rescued by antimycobacterial treatment, indicating that autophagy is required for effective antimycobacterial drug action in vivo. Moreover, activation of autophagy by antibiotic treatment dampened Mtb-induced proinflammatory responses in macrophages. Together, these findings underscore the importance of host autophagy in orchestrating successful antimicrobial responses to mycobacteria during chemotherapy.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/89906
DOI
10.1016/j.chom.2012.03.008
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://www.sciencedirect.com/science/article/pii/S1931312812001266
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse