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The promotion of cardiogenic differentiation of hMSCs by targeting epidermal growth factor receptor using microRNA-133a

 Se-Yeon Lee  ;  Onju Ham  ;  Min-Ji Cha  ;  Byeong-Wook Song  ;  Eunmi Choi  ;  Il-Kwon Kim  ;  Woochul Chang  ;  Soyeon Lim  ;  Chang Youn Lee  ;  Jun-Hee Park  ;  Jiyun Lee  ;  Yoonjin Bae  ;  Hyang-Hee Seo  ;  Eunhyun Choi  ;  Yangsoo Jang  ;  Ki-Chul Hwang 
 BIOMATERIALS, Vol.34(1) : 92-99, 2012 
Journal Title
Issue Date
Animals ; Base Sequence ; CHO Cells ; Cell Differentiation*/drug effects ; Cricetinae ; Humans ; Male ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism* ; Molecular Sequence Data ; Myocardial Infarction/physiopathology ; Myocardial Infarction/therapy ; Myocytes, Cardiac/cytology* ; Myocytes, Cardiac/drug effects ; Myocytes, Cardiac/metabolism ; Protein Kinase Inhibitors/pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptor, Epidermal Growth Factor/antagonists & inhibitors ; Receptor, Epidermal Growth Factor/metabolism* ; Stem Cell Transplantation
Mesenchymal stem cells ; Cardiogenic differentiation ; EGFR ; Compound 56 ; MicroRNA-133a
Human bone marrow-derived mesenchymal stem cells (hMSCs) are an attractive candidate for cell therapy in heart disease. Low survival and incomplete electromechanical integration between resident cardiomyocytes and transplanted hMSCs remain unsolved. In order for an infarcted heart to tolerate transplantation, differentiation capacity in stem cells must be reinforced. In this study, we found that compound 56, an epidermal growth factor receptor (EGFR) inhibitor, promotes cardiogenic differentiation of hMSCs and the transplantation of hMSCs treated with compound 56 resulted in enhancement of heart functions. Furthermore, hMSCs transfected with microRNA-133a (miR-133a), which targets EGFR, were observed to express cardiac-specific markers. We also discovered that luciferase activity is exclusively decreased by targeting EGFR in hMSCs transfected with miR-133a mimic. These results suggest that EGFR plays a key role in the regulation of cardiogenic differentiation in hMSCs.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Park, Jun-Hee(박준희)
Bae, Yoon Jin(배윤진)
Seo, Hyang Hee(서향희)
Song, Byeong Wook(송병욱)
Lee, Se Yeon(이세연)
Lee, Ji Yun(이지윤)
Lee, Chang Yeon(이창연)
Lim, So Yeon(임소연)
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Cha, Min Ji(차민지)
Choi, Eun Mi(최은미)
Choi, Eun Hyun(최은현)
Ham, On Ju(함온주)
Hwang, Ki Chul(황기철)
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