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Enhancement of MSC adhesion and therapeutic efficiency in ischemic heart using lentivirus delivery with periostin

 Yun-Hyeong Cho  ;  Min-Ji Cha  ;  Byeong-Wook Song  ;  Il-Kwon Kim  ;  Heesang Song  ;  Woochul Chang  ;  Soyeon Lim  ;  Onju Ham  ;  Se-Yeon Lee  ;  Eunmi Choi  ;  Hyuck Moon Kwon  ;  Ki-Chul Hwang 
 BIOMATERIALS, Vol.33(5) : 1376-1385, 2012 
Journal Title
Issue Date
Animals ; Apoptosis/drug effects ; Cell Adhesion/drug effects ; Cell Adhesion Molecules/metabolism* ; Cell Hypoxia/drug effects ; Cell Survival/drug effects ; Coculture Techniques ; Culture Media, Conditioned/pharmacology ; Gene Transfer Techniques* ; Lentivirus/genetics* ; Male ; Mesenchymal Stem Cell Transplantation* ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/metabolism ; Myocardial Ischemia/therapy* ; Myocardium/pathology ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/pathology ; Rats ; Rats, Sprague-Dawley ; Wound Healing/drug effects
Adhesion ; Infarcted myocardium ; Lentivirus ; Mesenchymal stem cells ; Periostin
Many approaches have shown beneficial effects of modified mesenchymal stem cells (MSCs) for treatment of infarcted myocardium, but have primarily focused on enhancing the survival of transplanted MSCs. Here, we show the dual benefits of periostin-overexpressing MSCs (p-MSCs) for infarcted myocardium. P-MSCs led to the marked histological and functional recovery of infarcted myocardium by enhancing survival of MSCs and directly preventing apoptosis of cardiomyocytes. Survival of p-MSCs themselves and cardiomyocytes co-cultured with p-MSCs or treated with the conditioned media from p-MSCs was significantly increased under hypoxic conditions. Decreases in adhesion-related integrins were reversed in cardiomyocytes co-cultured with p-MSCs, followed by increases in p-PI3K and Akt, indicating that periostin activates the PI3K pathway through adhesion-related integrins. When p-MSCs were injected into myocardial infarcted rats, histological pathology and cardiac function were significantly improved compared to MSC-injected controls. Thus, periostin might be a new target of therapeutic treatments using MSCs as carriers for infarcted myocardium.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kwon, Hyuck Moon(권혁문) ORCID logo https://orcid.org/0000-0001-9901-5015
Kim, Il Kwon(김일권)
Song, Byeong Wook(송병욱)
Lee, Se Yeon(이세연)
Cha, Min Ji(차민지)
Choi, Eun Mi(최은미)
Ham, On Ju(함온주)
Hwang, Ki Chul(황기철)
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