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Performance evaluation of new automated hepatitis B viral markers in the clinical laboratory: two quantitative hepatitis B surface antigen assays and an HBV core-related antigen assay.

Authors
 Yongjung Park  ;  Duck Jin Hong  ;  Saeam Shin  ;  Yonggeun Cho  ;  Hyon-Suk Kim 
Citation
 AMERICAN JOURNAL OF CLINICAL PATHOLOGY, Vol.137(5) : 770-777, 2012 
Journal Title
 AMERICAN JOURNAL OF CLINICAL PATHOLOGY 
ISSN
 0002-9173 
Issue Date
2012
MeSH
Biomarkers/blood ; Hepatitis B/diagnosis* ; Hepatitis B/immunology ; Hepatitis B/virology ; Hepatitis B Core Antigens/immunology* ; Hepatitis B Surface Antigens/immunology* ; Hepatitis B virus/immunology* ; Humans ; Pathology, Clinical/methods*
Keywords
Biomarkers/blood ; Hepatitis B/diagnosis* ; Hepatitis B/immunology ; Hepatitis B/virology ; Hepatitis B Core Antigens/immunology* ; Hepatitis B Surface Antigens/immunology* ; Hepatitis B virus/immunology* ; Humans ; Pathology, Clinical/methods*
Abstract
We evaluated quantitative hepatitis B surface antigen (qHBsAg) assays and a hepatitis B virus (HBV) core-related antigen (HBcrAg) assay. A total of 529 serum samples from patients with hepatitis B were tested. HBsAg levels were determined by using the Elecsys (Roche Diagnostics, Indianapolis, IN) and Architect (Abbott Laboratories, Abbott Park, IL) qHBsAg assays. HBcrAg was measured by using Lumipulse HBcrAg assay (Fujirebio, Tokyo, Japan). Serum aminotransferases and HBV DNA were respectively quantified by using the Hitachi 7600 analyzer (Hitachi High-Technologies, Tokyo, Japan) and the Cobas AmpliPrep/Cobas TaqMan test (Roche). Precision of the qHBsAg and HBcrAg assays was assessed, and linearity of the qHBsAg assays was verified. All assays showed good precision performance with coefficients of variation between 4.5% and 5.3% except for some levels. Both qHBsAg assays showed linearity from 0.1 to 12,000.0 IU/mL and correlated well (r = 0.9934). HBsAg levels correlated with HBV DNA (r = 0.3373) and with HBcrAg (r = 0.5164), and HBcrAg also correlated with HBV DNA (r = 0.5198; P < .0001). This observation could provide impetus for further research to elucidate the clinical usefulness of the qHBsAg and HBcrAg assays.
Files in This Item:
T201203469.pdf Download
DOI
22523216
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정)
Cho, Yonggeun(조용근) ORCID logo https://orcid.org/0000-0003-1946-4318
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89414
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