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Modified cytotoxicity evaluation of elastomeric impression materials while polymerizing with reduced exposure time

Authors
 Jae-Sung Kwon  ;  Sang-Bae Lee  ;  Chong-Kwan Kim  ;  Kyoung-Nam Kim 
Citation
 ACTA ODONTOLOGICA SCANDINAVICA, Vol.70(6) : 597-602, 2012 
Journal Title
 ACTA ODONTOLOGICA SCANDINAVICA 
ISSN
 0001-6357 
Issue Date
2012
MeSH
Biocompatible Materials* ; Dental Materials*
Keywords
biocompatibility ; cytotoxicity evaluation ; dental materials ; elastomeric impression materials
Abstract
OBJECTIVE: Cytotoxicity evaluation is an important step in biocompatibility assessment of dental impression materials. Previously, cytotoxicity evaluations were carried out on already set ('set') impression materials for contact time or extraction time of 24 h or longer in duration. However, clinically, dental impression materials are in contact with oral tissue while they are being set ('polymerizing'), for no longer than 10 min. Hence, the aim of this study was to investigate the difference in results between 'polymerizing' and 'set' forms of impression materials as well as the difference in results between longer duration of contact or extraction time (12 or 24 h) and shorter duration of time (15 or 30 min). MATERIALS AND METHODS: Seven dental impression materials of different chemical compositions were tested. Two commonly used in vitro tests were used-test on extraction and test by direct contact. Both 'polymerizing' and 'set' forms of impression materials were used with different durations of extraction and contact (15 min, 30 min, 12 h and 24 h). RESULTS: There were significant (p < 0.05) differences of cell viability and cell proliferation between the 'polymerizing' and 'set' impression materials. Also, significant (p < 0.05) differences were noted with variance in duration of time. CONCLUSION: In light of the results, it is recommended to use a 'polymerizing' state of dental impression material for cytotoxicity evaluation, with 15 or 30 min of contact between cell and dental impression materials and an extraction time of 15 or 30 min that is more reflective of clinical situations.
Full Text
http://informahealthcare.com/doi/abs/10.3109/00016357.2011.641127
DOI
22182313
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kyoung Nam(김경남)
Kim, Chong Kwan(김종관)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89374
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