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Culture on a 3,4-Dihydroxy-l-Phenylalanine-Coated Surface Promotes the Osteogenic Differentiation of Human Mesenchymal Stem Cells

Authors
 Wan-Geun La  ;  Jung-Youn Shin  ;  Suk Ho Bhang  ;  Min Jin  ;  Hee Hun Yoon  ;  Seong-Seo Noh  ;  Gun-Il Im  ;  Chang-Sung Kim  ;  Byung-Soo Kim 
Citation
 TISSUE ENGINEERING PART A, Vol.19(9-10) : 1255-1263, 2013 
Journal Title
 TISSUE ENGINEERING PART A 
ISSN
 1937-3341 
Issue Date
2013
MeSH
Alkaline Phosphatase/metabolism ; Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cell Differentiation/drug effects ; Cell Differentiation/genetics ; Cells, Cultured ; Coated Materials, Biocompatible/chemistry* ; Coated Materials, Biocompatible/pharmacology ; Dihydroxyphenylalanine/chemistry* ; Humans ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects ; Osteocalcin/metabolism ; Osteogenesis/drug effects*
Keywords
Alkaline Phosphatase/metabolism ; Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cell Differentiation/drug effects ; Cell Differentiation/genetics ; Cells, Cultured ; Coated Materials, Biocompatible/chemistry* ; Coated Materials, Biocompatible/pharmacology ; Dihydroxyphenylalanine/chemistry* ; Humans ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects ; Osteocalcin/metabolism ; Osteogenesis/drug effects*
Abstract
The culture surface can affect the in vitro differentiation of stem cells. In this study, we investigated whether modifying the culture surface with 3,4-dihydroxy-l-phenylalanine (DOPA), an element of mussel adhesion protein, could enhance the in vitro osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMMSCs). hBMMSCs cultured on DOPA-coated plates exhibited better cell adhesion and spreading compared with noncoated conventional tissue culture plates. The DOPA coated did not affect the apoptosis or viability of the cultured hBMMSCs. Also, hBMMSCs cultured on DOPA-coated plates exhibited a higher degree of osteogenic differentiation than did hBMMSCs cultured on noncoated plates, as evaluated with alkaline phosphate (ALP) activity, calcium content, and the mRNA expression of runt-related transcription factor 2, ALP, and osteocalcin. Further, hBMMSCs cultured on DOPA-coated plates demonstrated a higher capability of ectopic bone formation in vivo following implantation in the subcutaneous space of athymic mice compared with hBMMSCs cultured on noncoated plates, as evaluated with microcomputer topography analysis and histomorphometry. These results indicate that modifying the culture surface with DOPA can enhance the in vitro osteogenic differentiation of hBMMSCs.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/ten.tea.2012.0165
DOI
10.1089/ten.tea.2012.0165
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89203
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