Cited 7 times in
Culture on a 3,4-Dihydroxy-l-Phenylalanine-Coated Surface Promotes the Osteogenic Differentiation of Human Mesenchymal Stem Cells
DC Field | Value | Language |
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dc.contributor.author | 김창성 | - |
dc.date.accessioned | 2014-12-18T09:59:13Z | - |
dc.date.available | 2014-12-18T09:59:13Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89203 | - |
dc.description.abstract | The culture surface can affect the in vitro differentiation of stem cells. In this study, we investigated whether modifying the culture surface with 3,4-dihydroxy-l-phenylalanine (DOPA), an element of mussel adhesion protein, could enhance the in vitro osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMMSCs). hBMMSCs cultured on DOPA-coated plates exhibited better cell adhesion and spreading compared with noncoated conventional tissue culture plates. The DOPA coated did not affect the apoptosis or viability of the cultured hBMMSCs. Also, hBMMSCs cultured on DOPA-coated plates exhibited a higher degree of osteogenic differentiation than did hBMMSCs cultured on noncoated plates, as evaluated with alkaline phosphate (ALP) activity, calcium content, and the mRNA expression of runt-related transcription factor 2, ALP, and osteocalcin. Further, hBMMSCs cultured on DOPA-coated plates demonstrated a higher capability of ectopic bone formation in vivo following implantation in the subcutaneous space of athymic mice compared with hBMMSCs cultured on noncoated plates, as evaluated with microcomputer topography analysis and histomorphometry. These results indicate that modifying the culture surface with DOPA can enhance the in vitro osteogenic differentiation of hBMMSCs. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | TISSUE ENGINEERING PART A | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alkaline Phosphatase/metabolism | - |
dc.subject.MESH | Bone Marrow Cells/cytology | - |
dc.subject.MESH | Bone Marrow Cells/metabolism | - |
dc.subject.MESH | Cell Differentiation/drug effects | - |
dc.subject.MESH | Cell Differentiation/genetics | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Coated Materials, Biocompatible/chemistry* | - |
dc.subject.MESH | Coated Materials, Biocompatible/pharmacology | - |
dc.subject.MESH | Dihydroxyphenylalanine/chemistry* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology* | - |
dc.subject.MESH | Mesenchymal Stromal Cells/drug effects | - |
dc.subject.MESH | Osteocalcin/metabolism | - |
dc.subject.MESH | Osteogenesis/drug effects* | - |
dc.title | Culture on a 3,4-Dihydroxy-l-Phenylalanine-Coated Surface Promotes the Osteogenic Differentiation of Human Mesenchymal Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Periodontology (치주과학) | - |
dc.contributor.googleauthor | Wan-Geun La | - |
dc.contributor.googleauthor | Jung-Youn Shin | - |
dc.contributor.googleauthor | Suk Ho Bhang | - |
dc.contributor.googleauthor | Min Jin | - |
dc.contributor.googleauthor | Hee Hun Yoon | - |
dc.contributor.googleauthor | Seong-Seo Noh | - |
dc.contributor.googleauthor | Gun-Il Im | - |
dc.contributor.googleauthor | Chang-Sung Kim | - |
dc.contributor.googleauthor | Byung-Soo Kim | - |
dc.identifier.doi | 10.1089/ten.tea.2012.0165 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01041 | - |
dc.relation.journalcode | J02735 | - |
dc.identifier.eissn | 1937-335X | - |
dc.identifier.pmid | 23237247 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/ten.tea.2012.0165 | - |
dc.subject.keyword | Alkaline Phosphatase/metabolism | - |
dc.subject.keyword | Bone Marrow Cells/cytology | - |
dc.subject.keyword | Bone Marrow Cells/metabolism | - |
dc.subject.keyword | Cell Differentiation/drug effects | - |
dc.subject.keyword | Cell Differentiation/genetics | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Coated Materials, Biocompatible/chemistry* | - |
dc.subject.keyword | Coated Materials, Biocompatible/pharmacology | - |
dc.subject.keyword | Dihydroxyphenylalanine/chemistry* | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Mesenchymal Stromal Cells/cytology* | - |
dc.subject.keyword | Mesenchymal Stromal Cells/drug effects | - |
dc.subject.keyword | Osteocalcin/metabolism | - |
dc.subject.keyword | Osteogenesis/drug effects* | - |
dc.contributor.alternativeName | Kim, Chang Sung | - |
dc.contributor.affiliatedAuthor | Kim, Chang Sung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 9-10 | - |
dc.citation.startPage | 1255 | - |
dc.citation.endPage | 1263 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART A, Vol.19(9-10) : 1255-1263, 2013 | - |
dc.identifier.rimsid | 34445 | - |
dc.type.rims | ART | - |
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