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Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids

Authors
 Ju Hee Lee  ;  Jung U. Shin  ;  Inhee Jung  ;  Hemin Lee  ;  Dong Kyun Rah  ;  Jin Young Jung  ;  Won Jai Lee 
Citation
 BIOMED RESEARCH INTERNATIONAL, Vol.2013 : 621538, 2013 
Journal Title
 BIOMED RESEARCH INTERNATIONAL 
ISSN
 2314-6133 
Issue Date
2013
MeSH
Adult ; Aged ; Cells, Cultured ; Female ; Fibroblasts/metabolism* ; Fibroblasts/pathology ; Gene Expression Profiling* ; HSP70 Heat-Shock Proteins/biosynthesis* ; Humans ; Keloid/metabolism* ; Keloid/pathology ; Male ; Middle Aged ; Proliferating Cell Nuclear Antigen/biosynthesis ; Proteomics* ; Transforming Growth Factor beta/biosynthesis ; Up-Regulation*
Keywords
Adult ; Aged ; Cells, Cultured ; Female ; Fibroblasts/metabolism* ; Fibroblasts/pathology ; Gene Expression Profiling* ; HSP70 Heat-Shock Proteins/biosynthesis* ; Humans ; Keloid/metabolism* ; Keloid/pathology ; Male ; Middle Aged ; Proliferating Cell Nuclear Antigen/biosynthesis ; Proteomics* ; Transforming Growth Factor beta/biosynthesis ; Up-Regulation*
Abstract
Background. The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools. Objective. We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids. Methods. Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry. Results. Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts. Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts. Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF-β, and PCNA expressions in keloids compared to normal tissue. Conclusion. Hsp70 is overexpressed in keloid fibroblasts and tissue. The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids.
Files in This Item:
T201305283.pdf Download
DOI
10.1155/2013/621538
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Rah, Dong Kyun(나동균)
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88903
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