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Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D)

 Eun-Song Lee  ;  Si-Mook Kang  ;  Hae-Youn Ko  ;  Ho-Keun Kwon  ;  Baek-Il Kim 
 JOURNAL OF DENTISTRY, Vol.41(12) : 1264-1270, 2013 
Journal Title
Issue Date
Animals ; Bacterial Load ; Bacteriological Techniques ; Biofilms/growth & development* ; Cariogenic Agents/administration & dosage ; Cattle ; Color ; Culture Media ; Dental Caries/metabolism ; Dental Caries/microbiology* ; Dental Enamel/chemistry ; Dental Enamel/microbiology ; Dental Plaque/microbiology ; Fluorescence ; Hardness ; Humans ; Hydrogen-Ion Concentration ; Minerals/analysis ; Saliva/microbiology ; Saliva, Artificial ; Sucrose/administration & dosage
Biofilm ; Cariogenicity ; Dental caries ; Plaque ; Quantitative Light-induced Fluorescence-Digital ; Red fluorescence
OBJECTIVE: This study evaluated whether Quantitative Light-induced Fluorescence-Digital (QLF-D) can detect the levels of cariogenicity of dental microcosm biofilms by assessing the red fluorescence intensity. METHODS: Dental microcosm biofilms were initiated from human saliva on bovine enamel discs. Biofilms with various levels of cariogenicity were then grown in artificial saliva supplemented with sucrose at different concentrations (0.05%, 0.1%, 0.2%, and 0.5%) in 24-well microplates. After 10 days, fluorescence images of the biofilms were captured by the QLF-D to analyse the red fluorescence intensity, which was quantified as the red/green ratio (R/G value). The supernatant pH was also measured, as well as the total and aciduric bacteria counts of the collected biofilms. Mineral loss in enamel was also evaluated by calculating the percentage of surface microhardness changes (%SHC). RESULTS: The R/G values of the biofilms differed significantly with the sucrose concentration (p<0.0001), increasing consistently as the sucrose concentration increased from 0.05% (=0.91) to 0.5% (=2.56). Strong correlation was identified between the R/G value and the number of aciduric bacteria (r=0.83, p<0.0001), supernatant pH (r=-0.95, p<0.0001), and %SHC (r=0.90, p<0.0001). CONCLUSIONS: The red fluorescence as observed by the QLF-D was correlated with the cariogenic properties of dental microcosm biofilms in vitro, which indicates that this device can be used to detect the levels of cariogenicity of a dental biofilm. CLINICAL SIGNIFICANCE: The QLF-D is able to assess the cariogenic levels of dental plaque based on the intensity of red fluorescence.
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2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Si-Mook(강시묵) ORCID logo https://orcid.org/0000-0003-2341-3636
Ko, Hae Youn(고혜연)
Kwon, Ho Keun(권호근)
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
Lee, Eun Song(이은송) ORCID logo https://orcid.org/0000-0002-2949-4783
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