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Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D)

Authors
 Eun-Song Lee  ;  Si-Mook Kang  ;  Hae-Youn Ko  ;  Ho-Keun Kwon  ;  Baek-Il Kim 
Citation
 Journal of Dentistry, Vol.41(12) : 1264-1270, 2013 
Journal Title
 Journal of Dentistry 
ISSN
 0300-5712 
Issue Date
2013
Abstract
OBJECTIVE: This study evaluated whether Quantitative Light-induced Fluorescence-Digital (QLF-D) can detect the levels of cariogenicity of dental microcosm biofilms by assessing the red fluorescence intensity. METHODS: Dental microcosm biofilms were initiated from human saliva on bovine enamel discs. Biofilms with various levels of cariogenicity were then grown in artificial saliva supplemented with sucrose at different concentrations (0.05%, 0.1%, 0.2%, and 0.5%) in 24-well microplates. After 10 days, fluorescence images of the biofilms were captured by the QLF-D to analyse the red fluorescence intensity, which was quantified as the red/green ratio (R/G value). The supernatant pH was also measured, as well as the total and aciduric bacteria counts of the collected biofilms. Mineral loss in enamel was also evaluated by calculating the percentage of surface microhardness changes (%SHC). RESULTS: The R/G values of the biofilms differed significantly with the sucrose concentration (p<0.0001), increasing consistently as the sucrose concentration increased from 0.05% (=0.91) to 0.5% (=2.56). Strong correlation was identified between the R/G value and the number of aciduric bacteria (r=0.83, p<0.0001), supernatant pH (r=-0.95, p<0.0001), and %SHC (r=0.90, p<0.0001). CONCLUSIONS: The red fluorescence as observed by the QLF-D was correlated with the cariogenic properties of dental microcosm biofilms in vitro, which indicates that this device can be used to detect the levels of cariogenicity of a dental biofilm. CLINICAL SIGNIFICANCE: The QLF-D is able to assess the cariogenic levels of dental plaque based on the intensity of red fluorescence.
Full Text
http://www.sciencedirect.com/science/article/pii/S030057121300225X
DOI
10.1016/j.jdent.2013.08.021
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실)
Yonsei Authors
강시묵(Kang, Si-Mook) ORCID logo https://orcid.org/0000-0003-2341-3636
고혜연(Ko, Hae Youn)
권호근(Kwon, Ho Keun)
김백일(Kim, Baek Il) ORCID logo https://orcid.org/0000-0001-8234-2327
이은송(Lee, Eun Song) ORCID logo https://orcid.org/0000-0002-2949-4783
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88549
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