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Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D)

DC FieldValueLanguage
dc.contributor.author이은송-
dc.contributor.author강시묵-
dc.contributor.author고혜연-
dc.contributor.author권호근-
dc.contributor.author김백일-
dc.date.accessioned2014-12-18T09:38:24Z-
dc.date.available2014-12-18T09:38:24Z-
dc.date.issued2013-
dc.identifier.issn0300-5712-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/88549-
dc.description.abstractOBJECTIVE: This study evaluated whether Quantitative Light-induced Fluorescence-Digital (QLF-D) can detect the levels of cariogenicity of dental microcosm biofilms by assessing the red fluorescence intensity. METHODS: Dental microcosm biofilms were initiated from human saliva on bovine enamel discs. Biofilms with various levels of cariogenicity were then grown in artificial saliva supplemented with sucrose at different concentrations (0.05%, 0.1%, 0.2%, and 0.5%) in 24-well microplates. After 10 days, fluorescence images of the biofilms were captured by the QLF-D to analyse the red fluorescence intensity, which was quantified as the red/green ratio (R/G value). The supernatant pH was also measured, as well as the total and aciduric bacteria counts of the collected biofilms. Mineral loss in enamel was also evaluated by calculating the percentage of surface microhardness changes (%SHC). RESULTS: The R/G values of the biofilms differed significantly with the sucrose concentration (p<0.0001), increasing consistently as the sucrose concentration increased from 0.05% (=0.91) to 0.5% (=2.56). Strong correlation was identified between the R/G value and the number of aciduric bacteria (r=0.83, p<0.0001), supernatant pH (r=-0.95, p<0.0001), and %SHC (r=0.90, p<0.0001). CONCLUSIONS: The red fluorescence as observed by the QLF-D was correlated with the cariogenic properties of dental microcosm biofilms in vitro, which indicates that this device can be used to detect the levels of cariogenicity of a dental biofilm. CLINICAL SIGNIFICANCE: The QLF-D is able to assess the cariogenic levels of dental plaque based on the intensity of red fluorescence.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF DENTISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Load-
dc.subject.MESHBacteriological Techniques-
dc.subject.MESHBiofilms/growth & development*-
dc.subject.MESHCariogenic Agents/administration & dosage-
dc.subject.MESHCattle-
dc.subject.MESHColor-
dc.subject.MESHCulture Media-
dc.subject.MESHDental Caries/metabolism-
dc.subject.MESHDental Caries/microbiology*-
dc.subject.MESHDental Enamel/chemistry-
dc.subject.MESHDental Enamel/microbiology-
dc.subject.MESHDental Plaque/microbiology-
dc.subject.MESHFluorescence-
dc.subject.MESHHardness-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen-Ion Concentration-
dc.subject.MESHMinerals/analysis-
dc.subject.MESHSaliva/microbiology-
dc.subject.MESHSaliva, Artificial-
dc.subject.MESHSucrose/administration & dosage-
dc.titleAssociation between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D)-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Preventive Dentistry (예방치과학)-
dc.contributor.googleauthorEun-Song Lee-
dc.contributor.googleauthorSi-Mook Kang-
dc.contributor.googleauthorHae-Youn Ko-
dc.contributor.googleauthorHo-Keun Kwon-
dc.contributor.googleauthorBaek-Il Kim-
dc.identifier.doi10.1016/j.jdent.2013.08.021-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00049-
dc.contributor.localIdA00155-
dc.contributor.localIdA00268-
dc.contributor.localIdA00485-
dc.contributor.localIdA04635-
dc.relation.journalcodeJ01368-
dc.identifier.eissn1879-176X-
dc.identifier.pmid24012520-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S030057121300225X-
dc.subject.keywordBiofilm-
dc.subject.keywordCariogenicity-
dc.subject.keywordDental caries-
dc.subject.keywordPlaque-
dc.subject.keywordQuantitative Light-induced Fluorescence-Digital-
dc.subject.keywordRed fluorescence-
dc.contributor.alternativeNameLee, Eun Song-
dc.contributor.alternativeNameKang, Si Mook-
dc.contributor.alternativeNameKo, Hae Youn-
dc.contributor.alternativeNameKwon, Ho Keun-
dc.contributor.alternativeNameKim, Baek Il-
dc.contributor.affiliatedAuthorKang, Si Mook-
dc.contributor.affiliatedAuthorKo, Hae Youn-
dc.contributor.affiliatedAuthorKwon, Ho Keun-
dc.contributor.affiliatedAuthorKim, Baek Il-
dc.contributor.affiliatedAuthorLee, Eun Song-
dc.rights.accessRightsnot free-
dc.citation.volume41-
dc.citation.number12-
dc.citation.startPage1264-
dc.citation.endPage1270-
dc.identifier.bibliographicCitationJOURNAL OF DENTISTRY, Vol.41(12) : 1264-1270, 2013-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers

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