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Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model

Authors
 Jangwook Lee  ;  Min-Ji Cha  ;  Kwang Suk Lim  ;  Jang-Kyung Kim  ;  Sang-Kyung Lee  ;  Yong-Hee Kim  ;  Ki-Chul Hwang  ;  Kuen Yong Lee 
Citation
 JOURNAL OF DRUG TARGETING, Vol.21(9) : 822-829, 2013 
Journal Title
 JOURNAL OF DRUG TARGETING 
ISSN
 1061-186X 
Issue Date
2013
MeSH
Alginates/chemistry* ; Animals ; Apoptosis/drug effects ; Delayed-Action Preparations ; Disease Models, Animal ; Drug Carriers/chemistry* ; Gene Products, tat/administration & dosage ; Gene Products, tat/pharmacokinetics ; Gene Products, tat/therapeutic use* ; Glucuronic Acid/chemistry ; HSP27 Heat-Shock Proteins/administration & dosage ; HSP27 Heat-Shock Proteins/pharmacokinetics ; HSP27 Heat-Shock Proteins/therapeutic use* ; Hexuronic Acids/chemistry ; Hydrogels ; In Situ Nick-End Labeling ; Lactic Acid/chemistry* ; Male ; Microspheres ; Myocardial Infarction/drug therapy* ; Myocardial Infarction/metabolism ; Myocardial Infarction/pathology ; Polyglycolic Acid/chemistry* ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins/administration & dosage ; Recombinant Fusion Proteins/pharmacokinetics ; Recombinant Fusion Proteins/therapeutic use*
Keywords
Heat shock protein ; microsphere/hydrogel hybrid system ; myocardial infarction
Abstract
Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(d,l-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.
Full Text
http://informahealthcare.com/doi/abs/10.3109/1061186X.2013.829072
DOI
10.3109/1061186X.2013.829072
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Cha, Min Ji(차민지)
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88059
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