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Combination of CYP inhibitor with MEK/ERK inhibitor enhances the inhibitory effect on ERK in BRAF mutant colon cancer cells.

Authors
 SUN MIN LIM  ;  JEE WON HWANG  ;  JOONG BAE AHN  ;  SOO KYUNG BAE  ;  CHAN HEE PARK  ;  KI-YEOL KIM  ;  SUN YOUNG RHA  ;  HYUN CHEOL CHUNG  ;  JAE KYUNG ROH  ;  SANG JOON SHIN 
Citation
 Anticancer Research, Vol.33(6) : 2499-2508, 2013 
Journal Title
 Anticancer Research 
ISSN
 0250-7005 
Issue Date
2013
Abstract
BACKGROUND/AIM: To investigate mechanisms of discrepancy in response to a MEK/ERK inhibitor, U0126, in KRAS- and BRAF-mutant colorectal cancer cells. MATERIALS AND METHODS: Multiparametric flow cytometry was performed on two colon cancer cell lines, HCT116 and HT29. Cells were treated with U0126, and phospho-specific antibodies were used to monitor ERK signaling. RESULTS: HCT116 and HT29 cells were treated with increasing amounts of U0126. The western blot analysis revealed that by increasing the amount of U0126 resulted in inhibition of phospho-ERK, in HCT116 and to a lesser degree in HT29 cells. Microarray profiling identified CYP1A1 and 1A2 overexpression in HT29 cells and that inhibition of CYP1A1 with α-naphthoflavone and furanfylline restored sensitivity to U0126 in HT29 cells. CONCLUSION: Combination of a CYP inhibitor with MEK/ERK inhibitor enhances the inhibitory effect on ERK in BRAF-mutant colon cancer cells.
Full Text
http://ar.iiarjournals.org/content/33/6/2499.long
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
김기열(Kim, Ki Yeol) ORCID logo https://orcid.org/0000-0001-5357-1067
노재경(Roh, Jae Kyung)
라선영(Rha, Sun Young)
박찬희(Park, Chan Hee)
신상준(Shin, Sang Joon)
안중배(Ahn, Joong Bae)
임선민(Lim, Sun Min)
정현철(Chung, Hyun Cheol) ORCID logo https://orcid.org/0000-0002-0920-9471
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87808
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