1 270

Cited 15 times in

Effects of ozone exposure on the ocular surface

Authors
 Hun Lee  ;  Eung Kweon Kim  ;  Sang Won Kang  ;  Jae Hoon Kim  ;  Hye Jeong Hwang  ;  Tae-im Kim 
Citation
 Free Radical Biology and Medicine, Vol.63(SI) : 78-89, 2013 
Journal Title
 Free Radical Biology and Medicine 
ISSN
 0891-5849 
Issue Date
2013
MeSH
Active Transport, Cell Nucleus/drug effects ; Animals ; Cytokines/metabolism ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Eye/drug effects* ; Humans ; I-kappa B Proteins/metabolism ; Inflammation/genetics ; Inflammation/metabolism ; Mice ; NF-KappaB Inhibitor alpha ; NF-kappa B/genetics ; NF-kappa B/metabolism* ; Ocular Physiological Phenomena/drug effects ; Ocular Physiological Phenomena/genetics ; Ozone/administration & dosage* ; Phosphorylation ; Surface Properties/drug effects ; Tears/drug effects ; Tears/metabolism* ; Transcriptional Activation
Keywords
Free radicals ; Mucin-secreting cell ; NF-κB ; Ocular surface integrity ; Ozone ; Pyrrolidine dithiocarbamate ; Tear cytokines
Abstract
Changes in the ocular surface induced by ozone have received limited research attention. Here, we investigate the effects of ozone exposure on the integrity of the ocular surface, the production of inflammatory cytokines in tears, and changes in mucin-secreting cells in a mouse model. In addition, ozone-induced nuclear factor-κB (NF-κB)-mediated inflammatory processes were evaluated in cultured human conjunctival epithelial cells. In vivo, ozone induced the breakdown of corneal epithelial integrity, decreased the number of mucin-secreting cells, and induced the production of inflammatory cytokines, without altering tear volume. In vitro, ozone exposure led to increases in NF-κB nuclear translocation, κB-dependent transcriptional activity, NF-κB inhibitor α (IκBα) proteolysis, and expression of phosphorylated IκBα (p-IκBα), but did not cause cytotoxicity or cellular apoptosis. In addition, ozone induced the expression of inflammatory cytokines, Toll-like receptors, and C-C chemokine receptors, but decreased the expression of mucins. Furthermore, inhibition of NF-κB with pyrrolidine dithiocarbamate before exposure of cultured human conjunctival epithelial cells to ozone prevented changes in IκBα and p-IκBα levels in association with a decrease in the levels of inflammatory cytokines. Therefore, we conclude that ozone exposure interferes with ocular surface integrity and induces inflammation involving NF-κB-mediated processes at the level (and/or upstream) of IκBα. Understanding the role of ozone in the initiation of inflammatory processes on the animal ocular surface and in cultured human conjunctival epithelial cells can help elucidate the pathogenesis of ocular surface damage and suggest protective strategies for preserving a healthy ocular surface against ozone exposure.
Full Text
http://www.sciencedirect.com/science/article/pii/S0891584913002128
DOI
10.1016/j.freeradbiomed.2013.05.006
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Kim, Jae Hoon(김재훈) ORCID logo https://orcid.org/0000-0001-6599-7065
Kim, Tae Im(김태임) ORCID logo https://orcid.org/0000-0001-6414-3842
Hwang, Hye Jeong(황혜정) ORCID logo https://orcid.org/0000-0001-5144-3432
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87715
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse