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Therapeutic Effect of Oncolytic Adenovirus Expressing Relaxin in Radioresistant Oral Squamous Cell Carcinoma

Authors
 Lee, Sei Young  ;  Park, Haeng Ran  ;  Rhee, Junghoon  ;  Park, Young Min  ;  Kim, Se-Heon 
Citation
 ONCOLOGY RESEARCH, Vol.20(9) : 419-425, 2013 
Journal Title
 ONCOLOGY RESEARCH 
ISSN
 0965-0407 
Issue Date
2013
MeSH
Adenoviridae/genetics ; Adenoviridae/physiology* ; Animals ; Carcinoma, Squamous Cell/pathology* ; Carcinoma, Squamous Cell/secondary ; Cell Line, Tumor/radiation effects* ; Cell Line, Tumor/virology ; Female ; Genetic Therapy* ; Genetic Vectors/physiology* ; Humans ; Lymphatic Metastasis ; Mice ; Mice, Nude ; Mouth Neoplasms/pathology* ; Mutagenesis, Site-Directed ; Oncolytic Virotherapy* ; Oncolytic Viruses/genetics ; Oncolytic Viruses/physiology* ; Radiation Tolerance ; Relaxin/genetics ; Relaxin/physiology* ; Specific Pathogen-Free Organisms ; Tongue Neoplasms/pathology ; Xenograft Model Antitumor Assays
Keywords
Adenovirus ; Oral cancer ; Oral squamous cell carcinoma (OSCC) ; Radioresistance ; Relaxin (RLX)
Abstract
Radioresistance is one of the main determinants of treatment outcome in oral squamous cell carcinoma (OSCC), and treatment of radioresistant OSCC is difficult due to cross resistance to other conventional treatments. We aimed to identify whether genetically modified oncolytic adenovirus expressing relaxin (RLX), which affects collagen metabolism, can effectively inhibit growth of the radioresistant OSCC. Therapeutic effect of oncolytic adenovirus was compared between radiosensitive and radioresistant OSCC cell lines in vitro and in vivo, and spread of adenovirus throughout the tumor mass was verified by immunohistochemistry (IHC). Oncolytic adenovirus effectively killed cancer cells and there was no significant difference in the cytotoxic effect between radiosensitive and radioresistant OSCC cell lines. In animal experiments, the adenovirus significantly reduced the size of tumor, and there was no significant difference between radiosensitive and radioresistant OSCC. In IHC, RLX expressing adenovirus showed better proliferation and eliminated collagens more effectively compared to RLX nonexpressing adenovirus. These findings suggested that genetically modified oncolytic adenovirus can effectively inhibit growth of the radioresistant OSCC and might be a new therapeutic option in radioresistant OSCC.
Full Text
http://www.ingentaconnect.com/content/cog/or/2012/00000020/00000009/art00005?token=00531ed3e38ce7f208b0e567232d45232b45246c7b383b746656486b3568293c62207d673f582f6bec1
DOI
10.3727/096504013X13657689383139
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Heon(김세헌)
Park, Young Min(박영민) ORCID logo https://orcid.org/0000-0002-7593-8461
Park, Haeng Ran(박행란)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87393
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