2 262

Cited 15 times in

Isolation of mesenchymal stem-like cells in meningioma specimens

DC FieldValueLanguage
dc.contributor.author박은경-
dc.contributor.author심규원-
dc.contributor.author이지현-
dc.contributor.author장종희-
dc.contributor.author허용민-
dc.contributor.author강석구-
dc.contributor.author김동석-
dc.contributor.author김선호-
dc.contributor.author김세훈-
dc.contributor.author김의현-
dc.date.accessioned2014-12-18T08:57:13Z-
dc.date.available2014-12-18T08:57:13Z-
dc.date.issued2013-
dc.identifier.issn1019-6439-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/87259-
dc.description.abstractCells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105+ cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfInternational Journal of Oncology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdipogenesis/genetics-
dc.subject.MESHAnimals-
dc.subject.MESHAntigens, CD/metabolism-
dc.subject.MESHBone Marrow Cells/cytology*-
dc.subject.MESHBone Marrow Cells/metabolism-
dc.subject.MESHCell Differentiation/genetics*-
dc.subject.MESHCell Separation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChondrogenesis/genetics-
dc.subject.MESHEndoglin-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHHumans-
dc.subject.MESHMeningioma/genetics*-
dc.subject.MESHMeningioma/pathology-
dc.subject.MESHMesenchymal Stromal Cells/pathology*-
dc.subject.MESHMice-
dc.subject.MESHOsteogenesis/genetics-
dc.subject.MESHReceptors, Cell Surface/metabolism-
dc.titleIsolation of mesenchymal stem-like cells in meningioma specimens-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorHyo-Yeol Lim-
dc.contributor.googleauthorKyung Min Kim-
dc.contributor.googleauthorBo Kyung Kim-
dc.contributor.googleauthorJin-Kyoung Shim-
dc.contributor.googleauthorJi-Hyun Lee-
dc.contributor.googleauthorYong-Min Huh-
dc.contributor.googleauthorSe-Hoon Kim-
dc.contributor.googleauthorEui-Hyun Kim-
dc.contributor.googleauthorEun-Kyung Park-
dc.contributor.googleauthorKyu-Won Shim-
dc.contributor.googleauthorJong Hee Chang-
dc.contributor.googleauthorDong-Seok Kim-
dc.contributor.googleauthorSun Ho Kim-
dc.contributor.googleauthorYong-Kil Hong-
dc.contributor.googleauthorSu-Jae Lee-
dc.contributor.googleauthorSeok-Gu Kang-
dc.identifier.doi10.3892/ijo.2013.2053-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01607-
dc.contributor.localIdA02187-
dc.contributor.localIdA03470-
dc.contributor.localIdA04359-
dc.contributor.localIdA00036-
dc.contributor.localIdA00402-
dc.contributor.localIdA00560-
dc.contributor.localIdA00610-
dc.contributor.localIdA00837-
dc.contributor.localIdA03218-
dc.relation.journalcodeJ01141-
dc.identifier.pmid23921459-
dc.identifier.urlhttp://www.spandidos-publications.com/ijo/43/4/1260-
dc.subject.keywordAdipogenesis/genetics-
dc.subject.keywordAnimals-
dc.subject.keywordAntigens, CD/metabolism-
dc.subject.keywordBone Marrow Cells/cytology*-
dc.subject.keywordBone Marrow Cells/metabolism-
dc.subject.keywordCell Differentiation/genetics*-
dc.subject.keywordCell Separation-
dc.subject.keywordCells, Cultured-
dc.subject.keywordChondrogenesis/genetics-
dc.subject.keywordEndoglin-
dc.subject.keywordFlow Cytometry-
dc.subject.keywordHumans-
dc.subject.keywordMeningioma/genetics*-
dc.subject.keywordMeningioma/pathology-
dc.subject.keywordMesenchymal Stromal Cells/pathology*-
dc.subject.keywordMice-
dc.subject.keywordOsteogenesis/genetics-
dc.subject.keywordReceptors, Cell Surface/metabolism-
dc.contributor.alternativeNamePark, Eun Kyung-
dc.contributor.alternativeNameShim, Kyu Won-
dc.contributor.alternativeNameLee, Ji Hyun-
dc.contributor.alternativeNameChang, Jong Hee-
dc.contributor.alternativeNameHuh, Yong Min-
dc.contributor.alternativeNameKang, Seok Gu-
dc.contributor.alternativeNameKim, Dong Seok-
dc.contributor.alternativeNameKim, Sun Ho-
dc.contributor.alternativeNameKim, Se Hoon-
dc.contributor.alternativeNameKim, Eui Hyun-
dc.contributor.affiliatedAuthorPark, Eun Kyung-
dc.contributor.affiliatedAuthorShim, Kyu Won-
dc.contributor.affiliatedAuthorChang, Jong Hee-
dc.contributor.affiliatedAuthorHuh, Yong Min-
dc.contributor.affiliatedAuthorKang, Seok Gu-
dc.contributor.affiliatedAuthorKim, Dong Seok-
dc.contributor.affiliatedAuthorKim, Sun Ho-
dc.contributor.affiliatedAuthorKim, Se Hoon-
dc.contributor.affiliatedAuthorKim, Eui Hyun-
dc.contributor.affiliatedAuthorLee, Ji Hyun-
dc.rights.accessRightsnot free-
dc.citation.volume43-
dc.citation.number4-
dc.citation.startPage1260-
dc.citation.endPage1268-
dc.identifier.bibliographicCitationInternational Journal of Oncology, Vol.43(4) : 1260-1268, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.