Cited 19 times in
Isolation of mesenchymal stem-like cells in meningioma specimens
DC Field | Value | Language |
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dc.contributor.author | 박은경 | - |
dc.contributor.author | 심규원 | - |
dc.contributor.author | 이지현 | - |
dc.contributor.author | 장종희 | - |
dc.contributor.author | 허용민 | - |
dc.contributor.author | 강석구 | - |
dc.contributor.author | 김동석 | - |
dc.contributor.author | 김선호 | - |
dc.contributor.author | 김세훈 | - |
dc.contributor.author | 김의현 | - |
dc.date.accessioned | 2014-12-18T08:57:13Z | - |
dc.date.available | 2014-12-18T08:57:13Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87259 | - |
dc.description.abstract | Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105+ cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipogenesis/genetics | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, CD/metabolism | - |
dc.subject.MESH | Bone Marrow Cells/cytology* | - |
dc.subject.MESH | Bone Marrow Cells/metabolism | - |
dc.subject.MESH | Cell Differentiation/genetics* | - |
dc.subject.MESH | Cell Separation | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chondrogenesis/genetics | - |
dc.subject.MESH | Endoglin | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Meningioma/genetics* | - |
dc.subject.MESH | Meningioma/pathology | - |
dc.subject.MESH | Mesenchymal Stromal Cells/pathology* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Osteogenesis/genetics | - |
dc.subject.MESH | Receptors, Cell Surface/metabolism | - |
dc.title | Isolation of mesenchymal stem-like cells in meningioma specimens | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Hyo-Yeol Lim | - |
dc.contributor.googleauthor | Kyung Min Kim | - |
dc.contributor.googleauthor | Bo Kyung Kim | - |
dc.contributor.googleauthor | Jin-Kyoung Shim | - |
dc.contributor.googleauthor | Ji-Hyun Lee | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Se-Hoon Kim | - |
dc.contributor.googleauthor | Eui-Hyun Kim | - |
dc.contributor.googleauthor | Eun-Kyung Park | - |
dc.contributor.googleauthor | Kyu-Won Shim | - |
dc.contributor.googleauthor | Jong Hee Chang | - |
dc.contributor.googleauthor | Dong-Seok Kim | - |
dc.contributor.googleauthor | Sun Ho Kim | - |
dc.contributor.googleauthor | Yong-Kil Hong | - |
dc.contributor.googleauthor | Su-Jae Lee | - |
dc.contributor.googleauthor | Seok-Gu Kang | - |
dc.identifier.doi | 10.3892/ijo.2013.2053 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01607 | - |
dc.contributor.localId | A02187 | - |
dc.contributor.localId | A03470 | - |
dc.contributor.localId | A04359 | - |
dc.contributor.localId | A00036 | - |
dc.contributor.localId | A00402 | - |
dc.contributor.localId | A00560 | - |
dc.contributor.localId | A00610 | - |
dc.contributor.localId | A00837 | - |
dc.contributor.localId | A03218 | - |
dc.relation.journalcode | J01141 | - |
dc.identifier.eissn | 1791-2423 | - |
dc.identifier.pmid | 23921459 | - |
dc.identifier.url | http://www.spandidos-publications.com/ijo/43/4/1260 | - |
dc.subject.keyword | Adipogenesis/genetics | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Antigens, CD/metabolism | - |
dc.subject.keyword | Bone Marrow Cells/cytology* | - |
dc.subject.keyword | Bone Marrow Cells/metabolism | - |
dc.subject.keyword | Cell Differentiation/genetics* | - |
dc.subject.keyword | Cell Separation | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Chondrogenesis/genetics | - |
dc.subject.keyword | Endoglin | - |
dc.subject.keyword | Flow Cytometry | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Meningioma/genetics* | - |
dc.subject.keyword | Meningioma/pathology | - |
dc.subject.keyword | Mesenchymal Stromal Cells/pathology* | - |
dc.subject.keyword | Mice | - |
dc.subject.keyword | Osteogenesis/genetics | - |
dc.subject.keyword | Receptors, Cell Surface/metabolism | - |
dc.contributor.alternativeName | Park, Eun Kyung | - |
dc.contributor.alternativeName | Shim, Kyu Won | - |
dc.contributor.alternativeName | Lee, Ji Hyun | - |
dc.contributor.alternativeName | Chang, Jong Hee | - |
dc.contributor.alternativeName | Huh, Yong Min | - |
dc.contributor.alternativeName | Kang, Seok Gu | - |
dc.contributor.alternativeName | Kim, Dong Seok | - |
dc.contributor.alternativeName | Kim, Sun Ho | - |
dc.contributor.alternativeName | Kim, Se Hoon | - |
dc.contributor.alternativeName | Kim, Eui Hyun | - |
dc.contributor.affiliatedAuthor | Park, Eun Kyung | - |
dc.contributor.affiliatedAuthor | Shim, Kyu Won | - |
dc.contributor.affiliatedAuthor | Chang, Jong Hee | - |
dc.contributor.affiliatedAuthor | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Kang, Seok Gu | - |
dc.contributor.affiliatedAuthor | Kim, Dong Seok | - |
dc.contributor.affiliatedAuthor | Kim, Sun Ho | - |
dc.contributor.affiliatedAuthor | Kim, Se Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Eui Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Ji Hyun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 43 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1260 | - |
dc.citation.endPage | 1268 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF ONCOLOGY, Vol.43(4) : 1260-1268, 2013 | - |
dc.identifier.rimsid | 32931 | - |
dc.type.rims | ART | - |
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