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Survival of hypoxic human mesenchymal stem cells is enhanced by a positive feedback loop involving miR-210 and hypoxia-inducible factor 1

Authors
 Woochul Chang  ;  Chang Youn Lee  ;  Jun-Hee Park  ;  Moon-Seo Park  ;  Lee-So Maeng  ;  Chee Soon Yoon  ;  Min Young Lee  ;  Ki-Chul Hwang  ;  Yong-An Chung 
Citation
 JOURNAL OF VETERINARY SCIENCE, Vol.14(1) : 69-76, 2013 
Journal Title
JOURNAL OF VETERINARY SCIENCE
ISSN
 1229-845X 
Issue Date
2013
MeSH
Cell Survival ; Cobalt ; Gene Expression Regulation/physiology* ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/genetics ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism* ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/metabolism ; Mesenchymal Stromal Cells/physiology* ; MicroRNAs/metabolism* ; Oxygen/pharmacology ; Oxygen Consumption* ; RNA, Small Interfering/metabolism
Keywords
cell survival ; human mesenchymal stem cells ; hypoxia ; hypoxia inducible factor-1 ; microRNA-210
Abstract
The use of mesenchymal stem cells (MSCs) has emerged as a potential new treatment for myocardial infarction. However, the poor viability of MSCs after transplantation critically limits the efficacy of this new strategy. The expression of microRNA-210 (miR-210) is induced by hypoxia and is important for cell survival under hypoxic conditions. Hypoxia increases the levels of hypoxia inducible factor-1 (HIF-1) protein and miR-210 in human MSCs (hMSCs). miR-210 positively regulates HIF-1α activity. Furthermore, miR-210 expression is also induced by hypoxia through the regulation of HIF-1α. To investigate the effect of miR-210 on hMSC survival under hypoxic conditions, survival rates along with signaling related to cell survival were evaluated in hMSCs over-expressing miR-210 or ones that lacked HIF-1α expression. Elevated miR-210 expression increased survival rates along with Akt and ERK activity in hMSCs with hypoxia. These data demonstrated that a positive feedback loop involving miR-210 and HIF-1α was important for MSC survival under hypoxic conditions.
Files in This Item:
T201301604.pdf Download
DOI
23388440
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Park, Jun-Hee(박준희)
Lee, Chang Yeon(이창연)
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86911
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