169 334

Investigation of Oncogenic Cooperation in Simple Liver-Specific Transgenic Mouse Models Using Noninvasive In Vivo Imaging

Authors
 Hye-Lim Ju  ;  Sang Hoon Ahn  ;  Do Young Kim  ;  Sinhwa Baek  ;  Sook In Chung  ;  Jinsil Seong  ;  Kwang-Hyub Han  ;  Simon Weonsang Ro 
Citation
 PLoS One, Vol.8(3) : e59869, 2013 
Journal Title
 PLoS One 
ISSN
 1932-6203 
Issue Date
2013
Abstract
iver cancer is a complex multistep process requiring genetic alterations in multiple proto-oncogenes and tumor suppressor genes. Although hundreds of genes are known to play roles in hepatocarcinogenesis, oncogenic collaboration among these genes is still largely unknown. Here, we report a simple methodology by which oncogenic cooperation between cancer-related genes can be efficiently investigated in the liver. We developed various non-germline transgenic mouse models using hydrodynamics-based transfection which express HrasG12V, SmoM2, and a short-hairpin RNA down-regulating p53 (shp53) individually or in combination in the liver. In this transgenic system, firefly luciferase was co-expressed with the oncogenes as a reporter, allowing tumor growth in the liver to be monitored over time without an invasive procedure. Very strong bioluminescence imaging (BLI) signals were observed at 4 weeks post-hydrodynamic injection (PHI) in mice co-expressing HrasG12V and shp53, while only background signals were detected in other double or single transgenic groups until 30 weeks PHI. Consistent with the BLI data, tumors were observed in the HrasG12V plus shp53 group at 4 weeks PHI, while other transgenic groups failed to exhibit a hyperplastic nodule at 30 weeks PHI. In the HrasG12V plus shp53 transgenic group, BLI signals were well-correlated with actual tumor growth in the liver, confirming the versatility of BLI-based monitoring of tumor growth in this organ. The methodology described here is expected to accelerate and facilitate in vivo studies of the hepatocarcinogenic potential of cancer-related genes by means of oncogenic cooperation.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86896
Files in This Item:
T201301598.pdf Download
DOI
10.1371/journal.pone.0059869
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Liver Cirrhosis Clinical Research Center (간경변증임상연구센터)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
김도영(Kim, Do Young)
노원상(Ro, Simon Weonsang) ORCID logo https://orcid.org/0000-0003-2187-3698
백신화(Baek, Sin Hwa)
성진실(Seong, Jin Sil)
안상훈(Ahn, Sang Hoon)
정숙인(Chung, Sook In)
주혜림(Ju, Hye Lim)
한광협(Han, Kwang Hyup)
Export
RIS (EndNote)
XLS (Excel)
XML
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse