Cited 22 times in

Functional Screening of a Metagenomic Library Reveals Operons Responsible for Enhanced Intestinal Colonization by Gut Commensal Microbes

Authors
 Mi Young Yoon  ;  Kang-Mu Lee  ;  Yujin Yoon  ;  Junhyeok Go  ;  Yongjin Park  ;  Yong-Joon Cho  ;  Gerald W. Tannock  ;  Sang Sun Yoon 
Citation
 APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol.79(12) : 3829-3838, 2013 
Journal Title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN
 0099-2240 
Issue Date
2013
MeSH
Animals ; Bacterial Adhesion/genetics* ; Bacterial Adhesion/physiology ; Base Sequence ; Biofilms/growth & development* ; Chromosomes, Artificial, Bacterial/genetics ; Codon/genetics ; DNA Primers/genetics ; Escherichia coli/genetics* ; Escherichia coli/physiology ; Gene Library ; Intestine, Large/microbiology* ; Metagenome/genetics* ; Mice ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Open Reading Frames/genetics ; Operon/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Analysis, DNA
Keywords
Animals ; Bacterial Adhesion/genetics* ; Bacterial Adhesion/physiology ; Base Sequence ; Biofilms/growth & development* ; Chromosomes, Artificial, Bacterial/genetics ; Codon/genetics ; DNA Primers/genetics ; Escherichia coli/genetics* ; Escherichia coli/physiology ; Gene Library ; Intestine, Large/microbiology* ; Metagenome/genetics* ; Mice ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Open Reading Frames/genetics ; Operon/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Analysis, DNA
Abstract
Evidence suggests that gut microbes colonize the mammalian intestine through propagation as an adhesive microbial community. A bacterial artificial chromosome (BAC) library of murine bowel microbiota DNA in the surrogate hostEscherichia coliDH10B was screened for enhanced adherence capability. Two out of 5,472 DH10B clones, 10G6 and 25G1, exhibited enhanced capabilities to adhere to inanimate surfaces in functional screens. DNA segments inserted into the 10G6 and 25G1 clones were 52 and 41 kb and included 47 and 41 protein-coding open reading frames (ORFs), respectively. DNA sequence alignments, tetranucleotide frequency, and codon usage analysis strongly suggest that these two DNA fragments are derived from species belonging to the genusBacteroides. Consistent with this finding, a large portion of the predicted gene products were highly homologous to those ofBacteroidesspp. Transposon mutagenesis and subsequent experiments that involved heterologous expression identified two operons associated with enhanced adherence.E. colistrains transformed with the 10a or 25b operon adhered to the surface of intestinal epithelium and colonized the mouse intestine more vigorously than did the control strain. This study has revealed the genetic determinants of unknown commensals (probably resemblingBacteroidesspecies) that enhance the ability of the bacteria to colonize the murine bowel.
Files in This Item:
T201301280.pdf Download
DOI
10.1128/AEM.00581-13
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Yong Jin(박용진)
Yoon, Mi Young(윤미영)
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
Lee, Kang Mu(이강무) ORCID logo https://orcid.org/0000-0001-7414-5921
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86746
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links