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Detection and genotyping of human papillomavirus by five assays according to cytologic results

Authors
 Jonghyeon Choi  ;  Yongjung Park  ;  Eun Hee Lee  ;  Sinyoung Kim  ;  Jeong-Ho Kim  ;  Hyon-Suk Kim 
Citation
 JOURNAL OF VIROLOGICAL METHODS, Vol.187(1) : 79-84, 2013 
Journal Title
 JOURNAL OF VIROLOGICAL METHODS 
ISSN
 0166-0934 
Issue Date
2013
MeSH
Base Sequence ; DNA, Viral/analysis* ; DNA, Viral/genetics ; Female ; Genotype ; Human papillomavirus 16/genetics ; Human papillomavirus 18/genetics ; Humans ; Microarray Analysis ; Molecular Diagnostic Techniques/methods* ; Papillomaviridae/classification ; Papillomaviridae/genetics* ; Papillomavirus Infections/diagnosis* ; Papillomavirus Infections/genetics ; Papillomavirus Infections/virology ; Real-Time Polymerase Chain Reaction ; Sequence Analysis, DNA ; Uterine Cervical Neoplasms/virology
Keywords
Human papillomavirus (HPV) ; Genotyping ; Hybrid capture assay ; Real-time PCR ; Liquid beads microarray ; Peptide nucleic acid-based array
Abstract
Five assays for the detection of human papillomavirus (HPV) with different assay principles were evaluated. A total of 230 cervical swab specimens were collected from subjects according to the cytologic results. All specimens were tested by the following assays: hybrid capture 2 (HC2), two real-time PCR assays (Abbott RealTime HR and AdvanSure RealTime), liquid beads microarray (GeneFinder) and peptide nucleic acid-based array (PANArray). The HPV DNA of 99 samples was sequenced to identify genotypes. Concordance rates between the results for the identification of 14 high risk HPV genotypes by any two of the evaluated assays, except for AdvanSure RealTime, ranged from 83.0% to 88.3%, and those for the identification of genotypes 16 and 18, except for HC2, were 93.0% and 96.1%, respectively. The results for the evaluation of high risk HPV genotypes by HC2 agreed with those of the other assays in 76.5–86.5% of cases. Identification of HPV genotype by GeneFinder and PANArray corresponded with that by direct sequencing in 88.9% and 84.8% of sequenced samples. This study demonstrated that HC2 and the two real-time PCR assays could be used for routine HPV screening, and the other genotyping assays can be applied for epidemiologic surveillance.
Full Text
http://www.sciencedirect.com/science/article/pii/S0166093412003163
DOI
10.1016/j.jviromet.2012.09.005
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Jeong Ho(김정호) ORCID logo https://orcid.org/0000-0003-2479-0548
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86282
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