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Agmatine promotes the migration of murine brain endothelial cells via multiple signaling pathways

 Hyun-Joo Jung  ;  Yong-Heui Jeon  ;  Kiran Kumar Bokara  ;  Bon-Nyeo Koo  ;  Won Taek Lee  ;  Kyung Ah Park  ;  Jong-Eun Lee 
 LIFE SCIENCES, Vol.92(1) : 42-50, 2013 
Journal Title
Issue Date
Agmatine/administration & dosage ; Agmatine/pharmacology* ; Animals ; Blotting, Western ; Brain/cytology ; Brain/drug effects* ; Brain/metabolism ; Cell Movement/drug effects* ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelial Cells/drug effects* ; Endothelial Cells/metabolism ; Intercellular Adhesion Molecule-1/metabolism ; Mice ; Nitric Oxide/metabolism ; Signal Transduction/drug effects* ; Vascular Endothelial Growth Factor A/metabolism ; Vascular Endothelial Growth Factor Receptor-2/metabolism
Agmatine ; Migration ; ICAM-1 ; bEnd.3 cells ; VEGF ; NO
AIMS: The combination of adhesion and migration of endothelial cells (ECs) is an integral process for evolution, organization, repair and vessel formation in living organisms. Agmatine, a polycationic amine existing in brain, has been investigated to exert neuroprotective effects. Up to date, there are no studies reporting that agmatine modulates murine brain endothelial (bEnd.3) cells migration. In the present study, we intend to investigate the role of agmatine in bEnd.3 cells migration and the molecular mechanism mediating this action. MAIN METHODS: The effect of agmatine on the bEnd.3 cells migration was examined by migration assay, and the mechanism involved for this effect was investigated by western blot analysis and NO contents measurements. KEY FINDINGS: Agmatine treatment (50, 100 and 200 μM) significantly accelerated bEnd.3 cells migration in a concentration-dependent manner. Western blotting revealed that agmatine treatment significantly induced vascular endothelial growth factor (VEGF), VEGF receptor 2 (Flk-1/KDR or VEGFR2), phosphatidylinositol 3-kinase (PI3K), Akt/protein kinase B (also known as PKB, PI3K downstream effector protein), endothelial nitric oxide synthase (eNOS) nitric oxide (NO; product by eNOS) and intercellular adhesion molecule 1 (ICAM-1) expressions during bEnd.3 cells migration. The expression of ICAM-1 and migration of bEnd.3 cells, induced by agmatine, were significantly attenuated by treatment of wortmannin, a specific PI3K inhibitor. SIGNIFICANCE: Taken together, we provide the first evidence that activation of VEGF/VEGFR2 and the consequential PI3K/Akt/eNOS/NO/ICAM-1 signaling pathways are serial events, through which the treatment of agmatine could lead to bEnd.3 cells migration.
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1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Koo, Bon-Nyeo(구본녀) ORCID logo https://orcid.org/0000-0002-3189-1673
Park, Kyung Ah(박경아)
Lee, Won Taek(이원택) ORCID logo https://orcid.org/0000-0001-7348-9562
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
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