Cited 0 times in

144 7

A one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples

Authors
 Hwan Young Lee ; Jung Ah Yoon ; Kyoung-Jin Shin ; Woo Ick Yang 
Citation
 Legal Medicine, Vol.15(1) : 50~54, 2013 
Journal Title
 Legal Medicine 
ISSN
 1344-6223 
Issue Date
2013
Abstract
The mitochondrial DNA (mtDNA) haplogroup typing has become an essential tool to study human evolutionary history and to infer the matrilineal bio-geographic ancestry. In forensic field, the screening of mtDNA haplogroups by genotyping of mtDNA single nucleotide polymorphisms (SNPs) can help guarantee the quality of mtDNA sequence data as well as can reduce the need to sequence samples that do not match. Here, a multiplex mutagenically separated (MS) polymerase chain reaction (PCR) system was developed for simultaneous rapid detection of 14 coding region SNPs and one deletion motif representing common mtDNA haplogroups of East Asia. The multiplex MS PCR system we developed has the advantage of being a one step procedure that requires only a single PCR amplification with allele-specific primers and allowing straightforward designation of haplogroups along the branches of the phylogenetic tree. Therefore, it would be a simple, rapid, and reliable detection method useful for large-scale screening of mtDNA variations to determine East Asian mtDNA haplogroups.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86146
DOI
10.1016/j.legalmed.2012.08.002
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Forensic Medicine
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://www.sciencedirect.com/science/article/pii/S1344622312001332
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse