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A one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples

Authors
 Hwan Young Lee  ;  Jung Ah Yoon  ;  Woo Ick Yang  ;  Kyoung-Jin Shin 
Citation
 LEGAL MEDICINE, Vol.15(1) : 50-54, 2013 
Journal Title
LEGAL MEDICINE
ISSN
 1344-6223 
Issue Date
2013
MeSH
Asian Continental Ancestry Group/genetics* ; DNA Fingerprinting/methods* ; DNA, Mitochondrial/genetics* ; Forensic Genetics/methods* ; Genetics, Population* ; Haplotypes ; Humans ; Multiplex Polymerase Chain Reaction ; Republic of Korea
Keywords
Mitochondrial DNA haplogroup ; Single nucleotide polymorphism ; East Asian ; Mutagenically separated polymerase chain reaction
Abstract
The mitochondrial DNA (mtDNA) haplogroup typing has become an essential tool to study human evolutionary history and to infer the matrilineal bio-geographic ancestry. In forensic field, the screening of mtDNA haplogroups by genotyping of mtDNA single nucleotide polymorphisms (SNPs) can help guarantee the quality of mtDNA sequence data as well as can reduce the need to sequence samples that do not match. Here, a multiplex mutagenically separated (MS) polymerase chain reaction (PCR) system was developed for simultaneous rapid detection of 14 coding region SNPs and one deletion motif representing common mtDNA haplogroups of East Asia. The multiplex MS PCR system we developed has the advantage of being a one step procedure that requires only a single PCR amplification with allele-specific primers and allowing straightforward designation of haplogroups along the branches of the phylogenetic tree. Therefore, it would be a simple, rapid, and reliable detection method useful for large-scale screening of mtDNA variations to determine East Asian mtDNA haplogroups.
Full Text
http://www.sciencedirect.com/science/article/pii/S1344622312001332
DOI
10.1016/j.legalmed.2012.08.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Yoon, Jung Ah(윤정아)
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86146
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