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A one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples

DC Field Value Language
dc.contributor.author신경진-
dc.contributor.author양우익-
dc.contributor.author윤정아-
dc.contributor.author이환영-
dc.date.accessioned2014-12-18T08:21:08Z-
dc.date.available2014-12-18T08:21:08Z-
dc.date.issued2013-
dc.identifier.issn1344-6223-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/86146-
dc.description.abstractThe mitochondrial DNA (mtDNA) haplogroup typing has become an essential tool to study human evolutionary history and to infer the matrilineal bio-geographic ancestry. In forensic field, the screening of mtDNA haplogroups by genotyping of mtDNA single nucleotide polymorphisms (SNPs) can help guarantee the quality of mtDNA sequence data as well as can reduce the need to sequence samples that do not match. Here, a multiplex mutagenically separated (MS) polymerase chain reaction (PCR) system was developed for simultaneous rapid detection of 14 coding region SNPs and one deletion motif representing common mtDNA haplogroups of East Asia. The multiplex MS PCR system we developed has the advantage of being a one step procedure that requires only a single PCR amplification with allele-specific primers and allowing straightforward designation of haplogroups along the branches of the phylogenetic tree. Therefore, it would be a simple, rapid, and reliable detection method useful for large-scale screening of mtDNA variations to determine East Asian mtDNA haplogroups.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfLEGAL MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAsian Continental Ancestry Group/genetics*-
dc.subject.MESHDNA Fingerprinting/methods*-
dc.subject.MESHDNA, Mitochondrial/genetics*-
dc.subject.MESHForensic Genetics/methods*-
dc.subject.MESHGenetics, Population*-
dc.subject.MESHHaplotypes-
dc.subject.MESHHumans-
dc.subject.MESHMultiplex Polymerase Chain Reaction-
dc.subject.MESHRepublic of Korea-
dc.titleA one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Forensic Medicine (법의학)-
dc.contributor.googleauthorHwan Young Lee-
dc.contributor.googleauthorJung Ah Yoon-
dc.contributor.googleauthorWoo Ick Yang-
dc.contributor.googleauthorKyoung-Jin Shin-
dc.identifier.doi10.1016/j.legalmed.2012.08.002-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02085-
dc.contributor.localIdA02300-
dc.contributor.localIdA02594-
dc.contributor.localIdA03335-
dc.relation.journalcodeJ02161-
dc.identifier.eissn1873-4162-
dc.identifier.pmid22981178-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1344622312001332-
dc.subject.keywordMitochondrial DNA haplogroup-
dc.subject.keywordSingle nucleotide polymorphism-
dc.subject.keywordEast Asian-
dc.subject.keywordMutagenically separated polymerase chain reaction-
dc.contributor.alternativeNameShin, Kyoung Jin-
dc.contributor.alternativeNameYang, Woo Ick-
dc.contributor.alternativeNameYoon, Jung Ah-
dc.contributor.alternativeNameLee, Hwan Young-
dc.contributor.affiliatedAuthorShin, Kyoung Jin-
dc.contributor.affiliatedAuthorYang, Woo Ick-
dc.contributor.affiliatedAuthorYoon, Jung Ah-
dc.contributor.affiliatedAuthorLee, Hwan Young-
dc.rights.accessRightsnot free-
dc.citation.volume15-
dc.citation.number1-
dc.citation.startPage50-
dc.citation.endPage54-
dc.identifier.bibliographicCitationLEGAL MEDICINE, Vol.15(1) : 50-54, 2013-
dc.identifier.rimsid28811-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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