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Interferon gamma mRNA quantitative real-time polymerase chain reaction for the diagnosis of latent tuberculosis: a novel interferon gamma release assay

Authors
 Sunghyun Kim  ;  Young Keun Kim  ;  Hyejon Lee  ;  Jang-Eun Cho  ;  Hyo Youl Kim  ;  Young Uh  ;  Young Mi Kim  ;  Hyunjung Kim  ;  Sang-Nae Cho  ;  Bo-Young Jeon  ;  Hyeyoung Lee 
Citation
 Diagnostic Microbiology and Infectious Disease, Vol.75(1) : 68-72, 2013 
Journal Title
 Diagnostic Microbiology and Infectious Disease 
ISSN
 0732-8893 
Issue Date
2013
Abstract
The interferon gamma (IFN-γ) release assay (IGRA) is widely used as a diagnostic method for latent tuberculosis infection (LTBI). The QuantiFERON-TB Gold and QuantiFERON-TB Gold In-tube (QFT-IT) tests measure plasma IFN-γ levels using enzyme-linked immunosorbent assay (ELISA), and T-SPOT.TB counts IFN-γ–producing cells using enzyme-linked immunosorbent spot assay. IFN-γ mRNA was evaluated as an indicator of IGRA in comparison with QFT-IT IFN-γ ELISA in 46 subjects with active TB and in 73 at low risk for TB. Significant IFN-γ mRNA expression was detected from 30 min and peaked 4 h after stimulation with MTB antigens or mitogen. This was defined as the optimal time point for IFN-γ mRNA real-time polymerase chain reaction (PCR). The sensitivities of IFN-γ mRNA real-time PCR and IFN-γ ELISA were 84.8% (39/46) and 89.1% (41/46), respectively (no significant difference). Although the specificities of IFN-γ ELISA was 4.1% higher than that of IFN-γ mRNA real-time PCR (60.3% versus 56.2%), the difference was not statistically significant. The overall agreement between IFN-γ mRNA real-time PCR and IFN-γ ELISA was 79.8% (kappa = 0.475). Whilst there was no difference in the performance of IFN-γ mRNA real-time PCR and IFN-γ ELISA, IFN-γ mRNA real-time PCR was superior to IFN-γ ELISA in terms of the time required for detection of MTB infection.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86136
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889312003938
DOI
10.1016/j.diagmicrobio.2012.09.015
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
Yonsei Authors
조상래(Cho, Sang Nae)
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